Factor V dPCR dry Kit
The Factor V dPCR dry Kit genotypes the c.1601G>A variant (p.Arg534Gln) in the F5 gene, known as Factor V Leiden, in a single multiplexed reaction. The wild-type allele c.1601G is detected in the same reaction as a control for DNA quality. The master mix is supplied lyophilised, each reaction requires 30 ng of genomic DNA, and one kit covers 48 determinations. Research use only.
Quote-based, no public pricing. Add to your quote and our specialists put together a proposal with the right documentation, typically within 24 hours.
This kit in detail
- F5 c.1601G>A (p.Arg534Gln), Factor V Leiden
- Wild-type allele detected in the same reaction as a DNA quality control
- Multiplexed fluorophores: VIC for wild type, FAM for the variant
- 30 ng of genomic DNA per reaction, from peripheral blood
- Lyophilised master mix
- Heterozygous positive control included
- 48 determinations per kit
The Factor V dPCR dry Kit determines the genotype of the c.1601G>A variant (p.Arg534Gln) in the F5 gene, the variant known as Factor V Leiden, which is a risk factor for inherited thrombophilia.
Method
The assay uses oligonucleotides and fluorescent hydrolysis probes in a multiplexed reaction. The wild-type allele c.1601G is labelled with VIC and the variant allele with FAM, so both are read from the same reaction and the wild-type signal serves as a check on DNA quality. The master mix is supplied lyophilised, and the kit runs on systems with standard fluorescence channels.
Sample and input
The assay is intended for germline analysis on genomic DNA extracted from peripheral blood, and requires 30 ng of DNA per reaction.
Validation and controls
The assay was validated using reference DNA samples and synthetic DNA. An aqueous positive control that is heterozygous for the c.1601G>A transition is supplied.
Regulatory status
This kit is for research use only.
The kit contains the necessary reagents to perform 48 dPCR determinations. The reagents included in the kit are the following:
- Factor V Master Mix: Lyophilised master mix containing the oligonucleotides and hydrolysis probes for the multiplexed amplification of the wild type and the mutated genotype associated to Leiden. F5 wild type and F5 mutated genotype are labeled with fluorophores VIC and FAM, respectively.
- Factor V control: Aqueous positive control is heterozygous for the G- to -A transition at position c.1601 of the Leiden gene (c.1601G>A; p.Arg534Gin)
Datasheets and regulatory documentation
Everything your quality team needs for evaluation and procurement.
Instructions for Use
MSDS Factor V dPCR kit
Frequently paired with this kit
Other assays and instruments lab teams add alongside the Factor V dPCR dry Kit.
Add this kit to your quote
Tell us the platforms and throughput you’re planning for and our specialists will put together a clear proposal with the right documentation, no public pricing, just a considered answer.
