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The Familial Mediterranean Fever (FMF) PCR Detection Kit is a high-performance real-time PCR assay designed for the qualitative detection of MEFV gene mutations associated with FMF in human samples. Based on advanced hydrolysis probe (TaqMan®) qPCR technology, the assay enables sensitive and specific identification of the most clinically relevant FMF-associated polymorphisms.
Targeting key mutations within the MEFV gene, the kit supports accurate genetic diagnosis of this hereditary autoinflammatory disorder. An integrated internal control system monitors DNA quality and amplification efficiency, ensuring reliable and reproducible results.
With optimized assay chemistry, hot start technology, and compatibility with standard real-time PCR platforms, this kit provides a robust and efficient solution for molecular diagnostics and genetic research.
The FMF PCR Detection Kit is an in-vitro diagnostic real-time PCR assay developed for the qualitative identification of MEFV gene polymorphisms associated with Familial Mediterranean Fever. The assay utilizes hydrolysis probe technology to amplify and detect specific mutation targets with high sensitivity and specificity.
The kit includes multiple ready-to-use master mixes covering the most common MEFV mutations, along with internal controls to validate DNA quality and amplification performance.
Familial Mediterranean Fever (FMF) is an autosomal recessive autoinflammatory disorder caused by mutations in the MEFV gene, which encodes the protein pyrin. Pyrin plays a key role in regulating the innate immune response, particularly in controlling inflammatory pathways.
Mutations in MEFV result in dysregulated inflammation, leading to recurrent episodes of fever, serositis, arthritis, and characteristic skin manifestations. FMF predominantly affects populations from the Mediterranean region and can lead to serious complications such as amyloidosis if untreated.
Early and accurate genetic detection is essential for confirming diagnosis, guiding treatment decisions, and preventing long-term complications.
The FMF PCR Detection Kit is designed for reliable detection of multiple MEFV mutations in a single workflow. The assay employs real-time qPCR with hydrolysis probes to ensure precise mutation identification.
Hot start PCR technology minimizes non-specific amplification, while uracil-DNA glycosylase (UDG) reduces the risk of carry-over contamination. The internal control system verifies DNA integrity and detects potential PCR inhibition, supporting confident result interpretation.
Detection is based on fluorescence signals, where mutation-specific amplification is measured across designated channels, while the internal control confirms assay validity.
Instructions for Use