Helicobacter pylori PCR Kit
The Helicobacter pylori (H. pylori) PCR Detection Kit is a high-sensitivity real-time PCR assay designed for the qualitative detection of H. pylori DNA in human stool samples. Built on advanced hydrolysis probe (TaqMan) qPCR technology, the assay ensures highly sensitive and specific identification of H. pylori, a clinically significant gastrointestinal pathogen. Targeting conserved regions of the H. pylori genome, the kit provides reliable detection of the bacterium even in low-copy samples. An integrated internal control monitors DNA quality and PCR inhibition, ensuring accurate and confident results. With a detection sensitivity down to approximately 100 copies per reaction and a ready-to-use master mix optimized for major real-time PCR instruments, the H. pylori PCR Detection Kit is ideal for clinical laboratories and research settings requiring fast, accurate, and robust gastrointestinal pathogen detection.
Quote-based, no public pricing. Add to your quote and our specialists put together a proposal with the right documentation, typically within 24 hours.
This kit in detail
The Helicobacter pylori (H. pylori) PCR Detection Kit is a high-sensitivity real-time PCR assay designed for the qualitative detection of H. pylori DNA in human stool samples. Built on advanced hydrolysis probe (TaqMan) qPCR technology, the assay ensures highly sensitive and specific identification of H. pylori, a clinically significant gastrointestinal pathogen.
Targeting conserved regions of the H. pylori genome, the kit provides reliable detection of the bacterium even in low-copy samples. An integrated internal control monitors DNA quality and PCR inhibition, ensuring accurate and confident results.
With a detection sensitivity down to approximately 100 copies per reaction and a ready-to-use master mix optimized for major real-time PCR instruments, the H. pylori PCR Detection Kit is ideal for clinical laboratories and research settings requiring fast, accurate, and robust gastrointestinal pathogen detection.
- High sensitivity detection: 118 copies/test
- H. pylori DNA identification Dual-channel detection (FAM/HEX)
- High specificity for accurate
- Ready-to-use workflow for efficient laboratory processing
Qualitative PCR Detection of Helicobacter pylori
The H. pylori PCR Detection Kit is an in-vitro diagnostic real-time PCR assay developed for qualitative identification of H. pylori DNA in human stool samples. Utilizing hydrolysis probe technology, the assay delivers high sensitivity and specificity for this Gram-negative bacterium associated with gastritis, peptic ulcers, and gastric malignancies.
The kit includes a ready-to-use master mix containing primers and probes for both H. pylori detection and an internal control, enabling simultaneous verification of sample quality and amplification efficiency.
Molecular Background
Helicobacter pylori is a Gram-negative, spiral-shaped bacterium that colonizes the stomach lining and affects roughly 50% of the global population. Infection can persist asymptomatically but is associated with conditions such as peptic ulcer disease, non-ulcer dyspepsia, and nearly all cases of gastric MALT lymphoma. Accurate molecular detection is essential for effective diagnosis, patient management, and research into gastrointestinal disease.
Assay Performance & Utility
The H. pylori PCR Detection Kit provides reliable performance in routine diagnostic and research laboratories. The assay employs dual-channel fluorescence detection: the FAM channel specifically identifies H. pylori DNA, while the HEX channel functions as an internal control to confirm sample integrity and detect potential PCR inhibition.
Uracil-DNA glycosylase (UDG) is incorporated to prevent carry-over contamination from prior PCR runs, ensuring consistent and dependable results. Optimized assay chemistry and compatibility with major real-time PCR instruments enable rapid, accurate detection with minimal workflow complexity.
Technical Specifications
- Assay Type: Qualitative Real-Time PCR (qPCR)
- Target: Helicobacter pylori DNA
- Detection Channels: FAM (H. pylori), HEX (Internal Control)
- Sensitivity: 118 copies per test
- Sample Types: Fresh or frozen human stool samples
- Input Material: Extracted DNA
- Compatible Instruments: ABI 7500, QuantStudio 3/5/7, Bio-Rad CFX, Rotor-Gene Q, LightCycler 480, and equivalent systems
- H. pylori qPCR M. Mix
- H. pylori Positive Control
- Nuclease-free water
Datasheets and regulatory documentation
Everything your quality team needs for evaluation and procurement.
Instructions for Use
Frequently paired with this kit
Other assays and instruments lab teams add alongside the Helicobacter pylori PCR Kit.
Add this kit to your quote
Tell us the platforms and throughput you’re planning for and our specialists will put together a clear proposal with the right documentation, no public pricing, just a considered answer.
