Presto 100 CT/NG PCR Kit

Goffin Molecular Technologies: Pioneering Chlamydia Detection

Chlamydia remains one of the most diagnosed STIs worldwide. The urgency for rapid, precise, and trustworthy detection methods can’t be overstated. The Presto CT/NG stands as a paragon of innovation, fulfilling these very needs, a product of Goffin Molecular Technologies’ commitment to enhancing patient care through progressive diagnostic solutions.

Fundamental Principles of Presto’s Technology

Chlamydia trachomatis and Neisseria gonorrhoeae DNA detection via the PCR is rooted in amplifying specific segments of the CT cryptic plasmid DNA and the NG’s opa genes. The amplified PCR products are identified in real-time using specific internal probes, each connected to a distinct fluorescent reporter dye. This real-time tracking and advanced monitoring make the detection more precise and timely.

Unique Features Setting Presto Apart

Two standout features embellish the Presto Kit:

• Isolation/Inhibition Isolation Amplification Control: This component ensures that inhibitory substances in clinical samples, which might interfere with detection, are accounted for. By merging real-time PCR with this control, the kit provides a combination that ensures genuine positive results with high specificity and sensitivity.
• CT and NG Selectors: This component addresses potential issues with double infections wherein one bacterium’s high presence might overshadow the other’s detection. Through these selectors, the kit can identify even a low load of one target when a high load of another target exists in the same sample.

Presto’s Advantages: Why It’s A Game-Changer

For professionals in the field, the Presto CT/NG PCR Kit brings forward several compelling advantages:

• Fast Results: Capable of detecting all Chlamydia Trachomatis serovars and Neisseria Gonorrhoeae within just 60 minutes.
• High Sensitivity: Its real-time PCR foundation ensures top-notch sensitivity in detection.
• Exceptional Robustness: Crafted for reliability, making it the top pick for labs even in the most demanding and remote environments.
• Unparalleled Price Point: Manufactured in The Netherlands, it offers unparalleled quality at homebrew pricing, ensuring accessibility for labs globally.
• Unique Double Infection Detection: Employs selectors for accurate identification of double infections, capturing cases other kits might miss. Optimized Control: Incorporates a bacterial Isolation Amplification Control for comprehensive control of the PCR reaction.

The Final Word

The Presto 100 CT/NG PCR Kit from Goffin Molecular Technologies is not just another diagnostic tool; it represents the future of Chlamydia and Gonorrhea detection. For lab scientists, it offers a blend of speed, precision, and reliability that stands out in the crowded field of diagnostic solutions.

As Chlamydia detection methods evolve, it’s clear that the Presto CT/NG is paving the way, setting new standards, and offering labs an unparalleled tool in the battle against STIs.

 CT/NG PCR mix MASTER MIX (CG301001). 4 Tubes with a black colour code, labelled ”MASTER MIX” containing 400 µl ready to use PCR mix. This mixture contains four primers for amplification of the CT DNA, NG DNA and the Isolation Amplification Control DNA. It contains three fluorescent probes for detection of CT DNA, NG DNA and control DNA respectively. The master mix contains all ingredients for PCR amplification including DNA polymerase. 

CT/NG Isolation Amplification Control Isolation Amplification Control (CG301003). 4 Tubes with a red colour code, labelled ”Isolation Amplification Control” containing 150 µl Isolation Amplification Control. The Isolation Amplification Control consists of an inactivated E. coli modified with a genomic DNA fragment containing primer binding sites identical to the C. trachomatis and N. gonorrhoeae sequences, but with a different intermediate probe sequence. NOTE: resuspend before use. 

 CT Positive Control Positive Control CT (CG301005). 4 Tubes with a blue colour code, labelled “Positive Control CT” containing 55 µl positive control. The positive control consists of CT DNA at 4 IFU/ 10 µl. 

 NG Positive Control Positive Control NG (CG301006) 

4 tubes with a green colour code, labelled ”Positive Control NG” containing 55 µl positive control. The positive control consists of NG DNA at 100 CFU/ 10 µl. 

 CT/NG Negative Control Negative Control (CG301014). 2 Tubes with a transparent colour code, labelled ”Negative Control” containing 500 µl negative control.

CT Selector CT SELECTOR (CG301008). 1 Tube with a violet colour code, labelled ”CT SELECTOR” containing 50 µl of CT selector for use with high NG positive samples for the detection of a weak positive CT-co-infection.  

 NG Selector NG SELECTOR (CG301007). 1 Tube with a yellow colour code, labelled ‘NG SELECTOR’ containing 50 µl of NG selector for use with high CT positive samples for the detection of a weak positive NG-co-infection.