Hematology OncoKitDX
Hematology OncoKitDx is a next-generation sequencing panel for the genetic characterisation of haematologic neoplasms. From a single DNA sample it analyses 76 genes and 27 fusion genes, detects copy number variations across the genome, and identifies variants relevant to pharmacogenetics. One kit covers 24 reactions.
Quote-based, no public pricing. Add to your quote and our specialists put together a proposal with the right documentation, typically within 24 hours.
This kit in detail
- 76 genes and 27 fusion genes in one panel, covering targets from ABL1 to TCF3
- Copy number variation detection across the genome
- Pharmacogenetic variants relevant to chemotherapy response
- One DNA sample per patient for all targets
- 24 reactions per kit, with 24 unique 8-nucleotide indexes in single-use strips
- Positive controls and calculation tools included
Hematology OncoKitDx is a next-generation sequencing panel for the genetic characterisation of haematologic neoplasms. It analyses 76 genes and 27 fusion genes, covering targets from ABL1 to TCF3, and works from a single DNA sample.
Genes and fusion genes
Fusion genes are used in the diagnosis and classification of haematologic neoplasms and are difficult to detect with conventional methods. This panel covers them in the same run as the 76 target genes, so both variant types come from one analysis.
One DNA sample
All targets are analysed from a single DNA sample. A full genetic profile therefore does not require separate samples per marker, which shortens the workflow and reduces the number of samples needed per patient.
Copy number variations
Alongside sequence variants, the panel detects copy number variations across the genome. CNVs influence disease progression and treatment response, which makes them relevant when matching therapy to the genetic profile of a patient.
Pharmacogenetics
The panel detects variants related to pharmacogenetics, which affect a patient’s response to chemotherapy treatment. These results provide a basis for dose adjustment.
Gene selection
The gene content is aligned with international standards and with the availability of clinical trials.
Workflow
Library preparation uses Illumina adapters. The kit supplies 24 unique 8-nucleotide indexes in single-use strips, so 24 samples can be identified within one sequencing run. Positive controls and calculation tools for the analysis are included.
The kit contains the necessary reagents to perform 24 reactions. The reagents included in the kit are the following:
- Fragmentation Buffer: Buffer used for DNA fragmentation, prior to NGS libraries preparation.
- Fragmentation Enzyme: Enzyme used for DNA fragmentation, prior to NGS libraries preparation.
- Reagents Plate: Plate with all the reagents required to perform the DNA end repair, the Illumina adapters linkage, and amplifications done during the library preparation protocol.
- Beads and Buffers plate: Plate with magnetic particles and wash buffers, required for the DNA capture and its purification during the library preparation protocol.
- Index: Oligonucleotides with a unique 8 nucleotides identifier sequence compatible with the illumina adapters required to identify each sample during library preparation and NGS sequencing.
The kit includes 24 different indexes, prepared in single-use strips. - Hematology Probes Strips: Biotinylated synthetic oligonucleotides complementary to the target regions of the target genes.
- Elution Buffer: Buffer used to elute the DNA.
Datasheets and regulatory documentation
Everything your quality team needs for evaluation and procurement.
Instructions for Use
MSDS Hematology oncoKitDX
Frequently paired with this kit
Other assays and instruments lab teams add alongside the Hematology OncoKitDX.
Add this kit to your quote
Tell us the platforms and throughput you’re planning for and our specialists will put together a clear proposal with the right documentation, no public pricing, just a considered answer.
