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The Hepatitis A Virus (HAV) PCR Detection Kit is a high-sensitivity one-step real-time RT-qPCR assay designed for the detection of HAV RNA in human clinical samples. Utilizing advanced hydrolysis probe (TaqMan) technology, the assay enables highly specific and reliable identification of HAV, a major cause of acute viral hepatitis worldwide.
Targeting conserved regions of the HAV genome, the kit ensures accurate detection even in low viral load samples. An integrated internal control monitors RNA quality and detects potential PCR inhibition, ensuring confidence in diagnostic results.
With a detection sensitivity down to 10 copies per reaction and compatibility with widely used real-time PCR platforms, this kit is ideal for clinical diagnostics, outbreak management, and public health laboratories.
The HAV PCR Detection Kit is an in-vitro diagnostic one-step real-time RT-qPCR assay developed for the detection of HAV RNA in human samples. The assay combines reverse transcription and PCR amplification in a single reaction, delivering high sensitivity and specificity.
The kit includes a ready-to-use master mix containing primers and probes for both HAV detection and an internal control, enabling simultaneous verification of RNA quality and amplification efficiency.
Hepatitis A Virus (HAV) is a single-stranded RNA virus and one of the most common causes of acute viral hepatitis globally. Transmission primarily occurs via the fecal-oral route, often through contaminated food or water or close person-to-person contact.
Infection ranges from asymptomatic cases, particularly in children, to severe disease in adults, including fulminant hepatitis. Due to overlapping clinical symptoms with other hepatitis viruses, accurate molecular detection is essential for proper diagnosis and disease management.
The HAV PCR Detection Kit utilizes one-step RT-qPCR technology, enabling reverse transcription of viral RNA into cDNA followed by amplification and detection in a single tube. Detection is based on dual-channel fluorescence: the FAM channel identifies HAV RNA, while the HEX channel serves as an internal control to verify RNA integrity and detect PCR inhibition.
The assay incorporates “hot start” PCR technology to minimize non-specific amplification and enhance sensitivity. Uracil-DNA glycosylase (UDG) is included to prevent carry-over contamination, ensuring reliable and reproducible results.
With high analytical sensitivity and near 100% specificity under validated conditions, the kit enables accurate detection across a wide range of viral loads. Included quantification standards support assay calibration and performance verification.
Instructions for Use