Hepatitis C Virus (HCV) PCR Kit, tap a thumbnail to see more views.
Hepatitis C Virus (HCV) PCR Kit
The TruPCR HCV Viral Load Kit quantifies hepatitis C virus RNA in serum or plasma by real-time RT-PCR, across all genotypes and subtypes. The assay is calibrated against the WHO international standard and reports in international units per millilitre. Research use only. The stated limit of detection is 20 IU/ml. A five-point standard curve and an internal control that targets a human gene are included, so extraction and amplification efficiency are checked in every sample.
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This kit in detail
- Quantification of HCV RNA across all genotypes and subtypes
- Limit of detection 20 IU/ml, calibrated against the WHO standard
- Five-point standard curve, results in IU/ml
- Internal control on a human gene target
- Serum or plasma
- 24, 48 or 96 reactions per kit, REF 3B342, 3B296 and 3B297
The TruPCR HCV Viral Load Kit measures the hepatitis C viral load, the parameter that determines whether antiviral treatment is working. Research use only.
Clinical background
Hepatitis C virus is an RNA virus that causes chronic liver inflammation and, over years, fibrosis, cirrhosis and hepatocellular carcinoma. Direct-acting antivirals cure most infections, and the viral load is what shows whether the virus has been cleared. Because six genotypes circulate, an assay used for monitoring has to detect all of them equally.
Method
Real-time RT-PCR on a conserved region of the viral genome, with dual-channel detection: one channel for the target and one for the internal control, which targets a human gene sequence and so monitors extraction and amplification per sample. Quantification runs against a five-point standard curve calibrated to the WHO international standard, so results are reported in IU/ml. The stated limit of detection is 20 IU/ml. The kit contains the primer and probe mix, ready-to-use reverse transcription and amplification reagents and the quantitation controls.
Related product
For genotyping rather than quantification, the manufacturer offers the HCV Detection and Genotyping Kit, which distinguishes genotypes 1 to 6 (REF 3B253 and 3B254).
Regulatory status
This kit is for research use only.
Datasheets and regulatory documentation
Everything your quality team needs for evaluation and procurement.
Product Catalogus
Frequently paired with this kit
Other assays and instruments lab teams add alongside the Hepatitis C Virus (HCV) PCR Kit.
Add this kit to your quote
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