Respiratory Virus Detection Kit
The Respiratory Virus Detection Kit detects the genomic RNA of four respiratory viruses in one reaction: SARS-CoV-2, influenza A and B, and respiratory syncytial virus. Reverse transcription and real-time PCR run as a single one-step RT-qPCR procedure. Human RNase P is included as an endogenous control. One kit covers 96 reactions. Research use only.
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This kit in detail
- SARS-CoV-2 (FAM), influenza A and B (VIC) and RSV (Cy5) in one reaction
- Human RNase P as endogenous control (Texas Red)
- One-step RT-qPCR: reverse transcription and amplification in a single run
- Positive control with all four target sequences included
- 96 reactions per kit
The Respiratory Virus Detection Kit detects the genomic RNA of SARS-CoV-2, influenza A and B, and respiratory syncytial virus. Reverse transcription and real-time PCR detection of the target genes are combined in a single one-step RT-qPCR procedure, which reduces the number of handling steps per sample.
Targets and labelling
The master mix contains oligonucleotides and hydrolysis probes for SARS-CoV-2 labelled with FAM, influenza with VIC, RSV with Cy5 and the human endogenous control RNase P with Texas Red.
Endogenous control
Detection of the RNase P gene serves two purposes: it confirms that the RT-qPCR system performed as expected, and it supports interpretation of a qualitative result by showing that the sample contained amplifiable material.
Contents and throughput
The kit contains the Neumo master mix, an RT-PCR master mix, reverse transcriptase and a positive control with the target sequences for all four targets. One kit covers 96 reactions.
Regulatory status
This kit is for research use only.
The kit is equipped with reagents for 96 reactions, including:
- Neumo Master Mix: contains the oligonucleotides and hydrolysis probes to perform
system-specific amplification of SARS-CoV-2 (FAM), influenza (VIC), RSV (Cy5) and
human endogenous control, RNase P (Texas Red). - RT-PCR Master Mix: PCR Master Mix with the nucleotides, MgCl2, real-time PCR enzyme
and buffer required to perform real-time PCR. - RTase: retrotranscriptase enzyme to carry out the reverse transcription of RNA
to complementary DNA (cDNA). - Positive Control: positive control with the target sequences for the amplification
of the SARS-CoV-2, influenza, RSV and RNase P endogenous control targets.
Datasheets and regulatory documentation
Everything your quality team needs for evaluation and procurement.
Instructions for Use
MSDS Neumo ViralDx
Frequently paired with this kit
Other assays and instruments lab teams add alongside the Respiratory Virus Detection Kit.
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