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The Leptospira spp. PCR Detection Kit is a high-performance real-time PCR assay designed for the qualitative detection of Leptospira DNA in human samples. Utilizing advanced hydrolysis probe (TaqMan®) qPCR technology, the assay enables highly sensitive and specific identification of pathogenic Leptospira species.
Targeting conserved genomic regions, the kit provides reliable detection across a broad range of Leptospira strains. An integrated internal control system ensures validation of DNA quality and detection of potential PCR inhibition, supporting accurate and dependable results.
With optimized assay chemistry, hot start technology, and compatibility with major real-time PCR platforms, this kit offers an efficient solution for clinical diagnostics and infectious disease research.
The Leptospira spp. PCR Detection Kit is an in-vitro diagnostic real-time PCR assay developed for the qualitative identification of Leptospira DNA. The assay employs hydrolysis probe technology to amplify and detect specific bacterial DNA targets with high sensitivity and specificity.
The kit includes a ready-to-use master mix containing primers and probes for both Leptospira detection and an internal control gene, enabling simultaneous verification of sample quality and amplification efficiency.
Leptospira spp. are spiral-shaped Gram-negative bacteria (spirochetes) responsible for leptospirosis, a globally distributed zoonotic disease. The bacteria are commonly maintained in animal reservoirs, particularly rodents, and are transmitted to humans through exposure to contaminated water, soil, or urine.
Clinical manifestations range from mild flu-like symptoms to severe disease forms involving liver or kidney failure, haemorrhage, or meningitis. Due to the non-specific clinical presentation, accurate molecular detection is critical for timely diagnosis and appropriate patient management.
The assay is designed for reliable and rapid detection of Leptospira DNA in clinical samples such as whole blood and urine. Real-time PCR amplification ensures high analytical sensitivity, even in low bacterial load samples.
Hot start PCR technology minimizes non-specific amplification, while uracil-DNA glycosylase (UDG) prevents carry-over contamination. The dual-channel detection system enables clear interpretation of results: the FAM channel detects Leptospira DNA, while the HEX channel confirms internal control performance and sample integrity.
Instructions for Use