Leptospira spp. PCR Kit
The Leptospira spp. PCR Detection Kit is a high-performance real-time PCR assay designed for the qualitative detection of Leptospira DNA in human samples. Utilizing advanced hydrolysis probe (TaqMan®) qPCR technology, the assay enables highly sensitive and specific identification of pathogenic Leptospira species. Targeting conserved genomic regions, the kit provides reliable detection across a broad range of Leptospira strains. An integrated internal control system ensures validation of DNA quality and detection of potential PCR inhibition, supporting accurate and dependable results. With optimized assay chemistry, hot start technology, and compatibility with major real-time PCR platforms, this kit offers an efficient solution for clinical diagnostics and infectious disease research.
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This kit in detail
The Leptospira spp. PCR Detection Kit is a high-performance real-time PCR assay designed for the qualitative detection of Leptospira DNA in human samples. Utilizing advanced hydrolysis probe (TaqMan®) qPCR technology, the assay enables highly sensitive and specific identification of pathogenic Leptospira species.
Targeting conserved genomic regions, the kit provides reliable detection across a broad range of Leptospira strains. An integrated internal control system ensures validation of DNA quality and detection of potential PCR inhibition, supporting accurate and dependable results.
With optimized assay chemistry, hot start technology, and compatibility with major real-time PCR platforms, this kit offers an efficient solution for clinical diagnostics and infectious disease research.
- High sensitivity for early detection of Leptospira DNA
- High specificity across multiple Leptospira species
- Dual-channel detection with internal control (FAM/HEX)
- Ready-to-use workflow for efficient laboratory processing
Qualitative PCR Detection of Leptospira spp. DNA
The Leptospira spp. PCR Detection Kit is an in-vitro diagnostic real-time PCR assay developed for the qualitative identification of Leptospira DNA. The assay employs hydrolysis probe technology to amplify and detect specific bacterial DNA targets with high sensitivity and specificity.
The kit includes a ready-to-use master mix containing primers and probes for both Leptospira detection and an internal control gene, enabling simultaneous verification of sample quality and amplification efficiency.
Molecular Background
Leptospira spp. are spiral-shaped Gram-negative bacteria (spirochetes) responsible for leptospirosis, a globally distributed zoonotic disease. The bacteria are commonly maintained in animal reservoirs, particularly rodents, and are transmitted to humans through exposure to contaminated water, soil, or urine.
Clinical manifestations range from mild flu-like symptoms to severe disease forms involving liver or kidney failure, haemorrhage, or meningitis. Due to the non-specific clinical presentation, accurate molecular detection is critical for timely diagnosis and appropriate patient management.
Assay Performance & Utility
The assay is designed for reliable and rapid detection of Leptospira DNA in clinical samples such as whole blood and urine. Real-time PCR amplification ensures high analytical sensitivity, even in low bacterial load samples.
Hot start PCR technology minimizes non-specific amplification, while uracil-DNA glycosylase (UDG) prevents carry-over contamination. The dual-channel detection system enables clear interpretation of results: the FAM channel detects Leptospira DNA, while the HEX channel confirms internal control performance and sample integrity.
Technical Specifications
- Assay Type: Qualitative Real-Time PCR (qPCR)
- Target: Leptospira spp. DNA
- Detection Channels: FAM (Leptospira), HEX (Internal Control)
- Sensitivity: High analytical sensitivity
- Sample Types: Whole blood, urine
- Input Material: Extracted DNA
- Technology: One-step qPCR with hydrolysis probe detection
- Compatible Instruments: ABI 7500, QuantStudio series, Bio-Rad CFX, Rotor-Gene Q, LightCycler 480, and equivalent systems
- Leptospira spp. qPCR Master Mix
- Leptospira spp. Positive control
- Nuclease-free water
Datasheets and regulatory documentation
Everything your quality team needs for evaluation and procurement.
Instructions for Use
Frequently paired with this kit
Other assays and instruments lab teams add alongside the Leptospira spp. PCR Kit.
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