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Mastermix 16S Basic Kit

Short description

The Molzym Mastermix 16S Basic is a PCR amplification tool used to identify bacterial species in microbiome analysis. It minimizes contamination during the process and has high sensitivity and specificity. It is optimized to detect a broad range of bacterial species, even in low-abundance samples, and is easy to use with a two-step PCR protocol. The Mastermix 16S Basic is a valuable tool for laboratory professionals and scientists working in microbiome analysis, providing accurate and reliable results.

Product highlights

  • Minimizes contamination
  • High sensitivity and specificity
  • Detects low-abundance bacteria
  • Easy to use
  • DNA-free PCR enzyme
  • Provides accurate and reliable results

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Mastermix 16S Basic Kit

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Application Notes

 

Specifications of the Mastermix 16S Basic Kit

As a laboratory professional or scientist, you are probably familiar with the importance of accurate and reliable microbiome analysis. The Molzym Mastermix 16S Basic is a powerful tool that can aid in this process, providing precise results with minimal contamination.

The Mastermix 16S Basic is designed for the PCR amplification of bacterial 16S rRNA genes, which are commonly used in microbiome analysis. This method can identify the bacterial species present in a sample by sequencing the amplified 16S rRNA gene.

One of the key benefits of the Mastermix 16S Basic is its ability to minimize contamination during the PCR amplification process. Contamination can be a major issue in microbiome analysis, as it can lead to false positive results and inaccurate conclusions. The Mastermix 16S Basic utilizes a unique DNA-free PCR enzyme mix, which reduces the risk of contamination and ensures accurate results.

Another advantage of the Mastermix 16S Basic is its high sensitivity and specificity. The mix is optimized to detect a broad range of bacterial species, and it can accurately identify even low-abundance bacteria in complex microbiome samples. This can be particularly useful in clinical settings, where identifying specific bacterial infections is crucial.

The Mastermix 16S Basic is also easy to use, with a simple two-step PCR protocol. This can save time and reduce the potential for human error, allowing laboratory professionals to process samples more efficiently.

Overall, the Molzym Mastermix 16S Basic is a valuable tool for laboratory professionals and scientists working in microbiome analysis. Its ability to minimize contamination, high sensitivity and specificity, and ease of use make it an attractive option for accurate and reliable bacterial identification.

In conclusion, the Molzym Mastermix 16S Dye is a reliable, versatile, and sensitive tool that laboratory professionals and scientists can use for bacterial identification. Its unique intercalating dye technology, coupled with its high-throughput capabilities, make it an excellent choice for various applications, from clinical diagnostics to environmental monitoring. The kit provides a simple protocol that can be completed in a relatively short time, enabling researchers to detect and identify bacterial infections in a timely manner.

Components of the Mastermix 16S Basic Kit
  1. Mastermix 16S Basic, which contains all necessary PCR reagents and the unique DNA-free PCR enzyme mix
  2. Positive control DNA
  3. Negative control DNA
  4. Sterile water
  5. PCR tubes

 

The Mastermix 16S Basic is the main component of the kit and contains all the necessary reagents for PCR amplification of bacterial 16S rRNA genes. The positive control DNA and negative control DNA are provided to verify the performance of the kit and to detect any potential contamination. Sterile water is provided to dilute the DNA controls and to adjust the final reaction volume. PCR tubes are also included in the kit.

The kit does not include the template DNA or DNA-free water, which need to be added by the user. Additionally, the kit includes a comprehensive user manual that provides detailed instructions for the preparation and use of the Molzym Mastermix 16S Dye, as well as troubleshooting tips and advice on data interpretation.

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