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The Monkeypox (MPXV) PCR Detection Kit is a high-performance real-time PCR assay designed for the qualitative detection of Monkeypox virus DNA in human samples. Built on advanced hydrolysis probe-based qPCR technology, the assay ensures highly sensitive and specific identification of MPXV across a broad range of clinically relevant sample types, including blood, plasma, serum, and lesion swabs.
Targeting conserved regions of the MPXV genome, the kit enables reliable detection of circulating viral strains. An integrated internal control system is included to monitor DNA quality and detect potential PCR inhibition, ensuring accuracy and confidence in results.
With a detection sensitivity of up to 10 copies per reaction and optimized “hot start” chemistry to reduce non-specific amplification, the MPXV PCR Detection Kit is ideal for diagnostic laboratories requiring fast, accurate, and robust viral detection workflows. The assay is compatible with most standard real-time PCR instruments supporting FAM and HEX detection channels.
The MPXV PCR Detection Kit is an in-vitro diagnostic real-time PCR assay developed for the qualitative identification of Monkeypox virus DNA. The assay utilizes hydrolysis probe technology to amplify and detect specific viral gene targets, delivering high sensitivity and specificity.
The kit includes a ready-to-use master mix containing primers and probes for both MPXV detection and an internal control gene, enabling simultaneous validation of sample quality and amplification efficiency.
Monkeypox virus is a zoonotic Orthopoxvirus belonging to the Poxviridae family, closely related to Variola (smallpox), Vaccinia, and Cowpox viruses. It can be transmitted via animal-to-human or human-to-human contact, including exposure to infected lesions, bodily fluids, or respiratory droplets.
Clinically, MPXV infection presents with fever, lymphadenopathy, fatigue, and characteristic skin lesions that progress through multiple stages before healing. The incubation period ranges from 5 to 21 days, and the disease typically resolves within 2–4 weeks.
Accurate molecular detection is essential for outbreak control, differential diagnosis, and patient management, particularly due to symptom overlap with other viral infections.
The MPXV PCR Detection Kit is designed to deliver reliable performance in routine diagnostic and outbreak response settings. By combining high analytical sensitivity with robust assay design, the kit enables accurate detection of Monkeypox virus DNA even in low viral load samples.
The assay employs “hot start” PCR technology to reduce non-specific amplification and improve overall reaction efficiency. Additionally, uracil-DNA glycosylase (UDG) is incorporated to prevent carry-over contamination from previous PCR runs, ensuring consistent and dependable results.
Detection is based on a dual-channel fluorescence system, where the FAM channel specifically identifies the presence of Monkeypox DNA, while the HEX channel functions as an internal control to verify sample integrity and detect potential PCR inhibition. This built-in control system supports confident interpretation of both positive and negative results.
With a detection limit as low as 10 copies per reaction and near 100% specificity under validated conditions, the assay provides high diagnostic accuracy. Its compatibility with multiple sample types including blood, plasma, serum, and lesion material, makes it a versatile solution for clinical laboratories seeking efficient and dependable Monkeypox virus detection.
Instructions for Use