X
- No products in the list
The imegen® MTHFR II Polymorphism Kit is a molecular biology assay designed for the analysis of genetic variation in the MTHFR gene. It enables reliable detection of relevant variants using real-time PCR technology.
The kit is optimized for efficient workflows, requiring only 50 ng of genomic DNA, and includes a positive control to support consistent assay performance. It is compatible with standard real-time PCR platforms and supports reproducible results in molecular biology applications.
Note that this kit is for Research Use Only (RUO)
The imegen® MTHFR II Polymorphism Kit is designed for the analysis of genetic variation in the MTHFR gene using real-time PCR technology. It enables the detection of the c.1298A>C variant and the corresponding wild-type allele in a single assay.
The MTHFR gene, located on chromosome 1p36.22, encodes the enzyme methylenetetrahydrofolate reductase, which plays a role in folate metabolism and the remethylation of homocysteine to methionine. The c.1298A>C polymorphism is associated with altered enzyme activity and is widely studied in molecular biology research.
The kit is optimized for efficient laboratory workflows and requires 50 ng of genomic DNA, typically extracted from peripheral blood. It utilizes sequence-specific oligonucleotides and fluorescent hydrolysis probes for sensitive and specific detection. A synthetic positive control is included to support assay performance and result interpretation.
The assay is compatible with standard real-time PCR platforms, allowing straightforward integration into existing laboratory procedures.
The imegen® MTHFR II Polymorphism Kit provides a reliable solution for the analysis of genetic variation in the MTHFR gene. Its robust design supports consistent and reproducible results in molecular biology applications.
The kit contians the necessary reagents to perform 48 reactions. The reagents included in the kit are the following:
Instructions for Use
MSDS MTHFR II Polymorphism Kit
For any missing information or if you require additional details, please do not hesitate to contact us.