MTHFR II Polymorphism Kit
The imegen® MTHFR II Polymorphism Kit is a molecular biology assay designed for the analysis of genetic variation in the MTHFR gene. It enables reliable detection of relevant variants using real-time PCR technology. The kit is optimized for efficient workflows, requiring only 50 ng of genomic DNA, and includes a positive control to support consistent assay performance. It is compatible with standard real-time PCR platforms and supports reproducible results in molecular biology applications. Note that this kit is for Research Use Only (RUO)
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This kit in detail
The imegen® MTHFR II Polymorphism Kit is a molecular biology assay designed for the analysis of genetic variation in the MTHFR gene. It enables reliable detection of relevant variants using real-time PCR technology.
The kit is optimized for efficient workflows, requiring only 50 ng of genomic DNA, and includes a positive control to support consistent assay performance. It is compatible with standard real-time PCR platforms and supports reproducible results in molecular biology applications.
Note that this kit is for Research Use Only (RUO)
- Detection of the c.1298A>C variant and wild-type allele
- Real-time PCR with fluorescent probe technology
- Includes synthetic positive control
- Requires only 50 ng genomic DNA
MTHFR II Polymorphism Kit: Advanced Genetic Analysis
The imegen® MTHFR II Polymorphism Kit is designed for the analysis of genetic variation in the MTHFR gene using real-time PCR technology. It enables the detection of the c.1298A>C variant and the corresponding wild-type allele in a single assay.
Background
The MTHFR gene, located on chromosome 1p36.22, encodes the enzyme methylenetetrahydrofolate reductase, which plays a role in folate metabolism and the remethylation of homocysteine to methionine. The c.1298A>C polymorphism is associated with altered enzyme activity and is widely studied in molecular biology research.
Workflow and Application
The kit is optimized for efficient laboratory workflows and requires 50 ng of genomic DNA, typically extracted from peripheral blood. It utilizes sequence-specific oligonucleotides and fluorescent hydrolysis probes for sensitive and specific detection. A synthetic positive control is included to support assay performance and result interpretation.
The assay is compatible with standard real-time PCR platforms, allowing straightforward integration into existing laboratory procedures.
Conclusion
The imegen® MTHFR II Polymorphism Kit provides a reliable solution for the analysis of genetic variation in the MTHFR gene. Its robust design supports consistent and reproducible results in molecular biology applications.
The kit contians the necessary reagents to perform 48 reactions. The reagents included in the kit are the following:
- MTHFRII Master Mix: Specific oligonucleotides, labelled probes and water reagents for the
PCR reactions. - General Master Mix: Conventional PCR enzyme containing 10-30% glycerol, <0.1 sodium
azide and buffers. - Positive Control: Heterozygous positive control for the c.1298A>C (p. Glu433Ala) mutation
in MTHFR gene.
Datasheets and regulatory documentation
Everything your quality team needs for evaluation and procurement.
Instructions for Use
MSDS MTHFR II Polymorphism Kit
Frequently paired with this kit
Other assays and instruments lab teams add alongside the MTHFR II Polymorphism Kit.
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