OXA-23 Carbapenemase Rapid Test – EZER™
The EZER™ OXA-23 Carbapenemase Rapid Test is a lateral flow immunochromatographic assay for the qualitative detection of OXA-23 Carbapenemase in Acinetobacter spp. colonies. This test provides a fast, accurate, and easy-to-use solution to identify carbapenem-resistant strains-particularly A. baumannii-within just 15 minutes. OXA-23 Carbapenemase is a class D β-lactamase enzyme responsible for a major portion of carbapenem resistance in Acinetobacter baumannii. Early and reliable identification is critical for guiding infection control measures and antimicrobial therapy. Designed for clinical laboratories, the EZER™ test detects the presence of OXA-23 with a simple sample extraction and visual readout, eliminating the need for PCR or sequencing. The assay shows 100% sensitivity, specificity, and accuracy compared to molecular reference methods across 273 clinical isolates. The test is ready-to-use and contains pre-filled extraction buffer tubes and membrane-based test cassettes. A clear red line in the test (T) and control (C) zones indicates an OXA-23-positive result. It is ideal for infection control units, reference labs, and antimicrobial resistance surveillance.
Quote-based, no public pricing. Add to your quote and our specialists put together a proposal with the right documentation, typically within 24 hours.
This kit in detail
The EZER™ OXA-23 Carbapenemase Rapid Test is a lateral flow immunochromatographic assay for the qualitative detection of OXA-23 Carbapenemase in Acinetobacter spp. colonies. This test provides a fast, accurate, and easy-to-use solution to identify carbapenem-resistant strains-particularly A. baumannii-within just 15 minutes.
OXA-23 Carbapenemase is a class D β-lactamase enzyme responsible for a major portion of carbapenem resistance in Acinetobacter baumannii. Early and reliable identification is critical for guiding infection control measures and antimicrobial therapy.
Designed for clinical laboratories, the EZER™ test detects the presence of OXA-23 with a simple sample extraction and visual readout, eliminating the need for PCR or sequencing. The assay shows 100% sensitivity, specificity, and accuracy compared to molecular reference methods across 273 clinical isolates.
The test is ready-to-use and contains pre-filled extraction buffer tubes and membrane-based test cassettes. A clear red line in the test (T) and control (C) zones indicates an OXA-23-positive result. It is ideal for infection control units, reference labs, and antimicrobial resistance surveillance.
- OXA-23-targeted – Detects carbapenemase-producing Acinetobacter spp.
- Fast and reliable – Results in 15 minutes with 100% accuracy
- No instrumentation – Suitable for routine labs and AMR screening
- Clear readout – Red lines indicate positive/negative/inconclusive results
What is in the OXA-23 Carbapenemase Rapid Test?
The EZER™ test kit includes:
-
20 test devices (cassette format)
-
20 extraction tubes prefilled with buffer
-
1 package insert with instructions
The test cassette contains monoclonal antibodies against OXA-23 Carbapenemase, fixed on a nitrocellulose membrane. The buffer extract of a bacterial colony is applied, and if OXA-23 is present, red lines appear at both T and C regions.
Role of OXA-23 Carbapenemase in Resistance
OXA-23 Carbapenemase is a carbapenem-hydrolyzing class D β-lactamase (CHDL) frequently found in multidrug-resistant A. baumannii strains. This enzyme breaks down carbapenem antibiotics, rendering treatments ineffective.
It is strongly associated with:
-
Nosocomial infections in ICUs
-
Burn wound colonization
-
Postoperative infections
-
Ventilator-associated pneumonia
-
Catheter-associated urinary tract infections
Rapid detection of OXA-23-positive isolates enables:
-
Targeted antibiotic therapy
-
Infection prevention protocols
-
Outbreak containment
-
Monitoring of carbapenem resistance trend
About the EZER™ OXA-23 Carbapenemase Rapid Test
This immunochromatographic test uses monoclonal antibodies against OXA-23, labeled with colloidal gold. It provides visual results without instrumentation and is designed for rapid point-of-care or lab-based diagnosis.
Performance Characteristics:
-
Relative Sensitivity: 100%
-
Relative Specificity: 100%
-
Accuracy: 100%
-
Detection Limit: 0.1 ng/mL (recombinant OXA-23 protein)
Cross-Reactivity:
No cross-reactivity with other resistance markers such as:
-
OXA-24, OXA-48, OXA-58, OXA-181
-
NDM-1, KPC-2, VIM-1, CTX-M, SHV, TEM
-
MCR-1, AmpC, rmtB, sul1, tet(A), and more
Principle and Workflow
Sample Collection:
-
Touch 3 colonies from agar plate using a 1 µL loop
-
Suspend in provided extraction buffer tube
-
Vortex to homogenize
Testing Steps:
-
Open foil pouch and remove test cassette
-
Apply 3 drops (80 µL) of extracted sample to sample well
-
Wait 15 minutes and interpret results:
Interpretation:
-
Positive: Red lines at C and T
-
Negative: Red line at C only
-
Invalid: No line at C – retest with a new cassette
A positive line may appear within 3 minutes but full incubation is required for reliable negatives.
Clinical Application
The EZER™ OXA-23 Carbapenemase Rapid Test is ideal for:
-
Clinical microbiology labs screening A. baumannii
-
Infection control teams in hospital settings
-
AMR surveillance programs in healthcare systems
-
Reference laboratories in high-burden regions
By enabling rapid identification of OXA-23-positive organisms, this test supports:
-
Timely therapy adjustment
-
Early patient isolation
-
Limiting spread of carbapenem-resistant Acinetobacter
-
Reducing reliance on costly molecular diagnostics
Its ease of use and high specificity make it suitable even in lower-resource settings with high infection risk.
Datasheets and regulatory documentation
Everything your quality team needs for evaluation and procurement.
Frequently paired with this kit
Other assays and instruments lab teams add alongside the OXA-23 Carbapenemase Rapid Test – EZER™.
Add this kit to your quote
Tell us the platforms and throughput you’re planning for and our specialists will put together a clear proposal with the right documentation, no public pricing, just a considered answer.
