PIK3CA-E545G 50% AF FFPE Reference Standard curl
The PIK3CA-E545G 50% AF FFPE Reference Standard curl is a high-quality FFPE reference standard that mimics patient samples and is essential for clinical genomics and molecular diagnostics. Derived from human cell lines, this product is used in diagnostic and R&D labs for assay validation, protocol development, and instrument calibration. This reference curl features 2,287 cancer-relevant mutations across 116 genes, with PIK3CA-E545G mutation at a 50% allelic frequency confirmed via ddPCR. It supports: Reliable DNA/RNA extraction Enhanced control in sequencing and PCR workflows, including WGS and WES Determination of sensitivity limits Routine quality checks for research applications Each curl yields a minimum of 400 ng of DNA or RNA using the Qiagen AllPrep DNA/RNA FFPE kit, with a curl thickness of 10 µm and a shelf life of up to 24 months at 2-8°C. The PIK3CA-E545G 50% AF FFPE Reference Standard curl provides reliable quality control, enhancing reproducibility in genetic testing.
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This kit in detail
- Ensures reliable DNA/RNA extraction in genomics workflows.
- Provides accurate control for sequencing and PCR protocols.
- Determines sensitivity limits for diagnostic precision.
- Enables routine, consistent quality monitoring for research.
The PIK3CA-E545G 50% AF FFPE Reference Standard curl is a formalin-fixed paraffin-embedded (FFPE) reference material derived from human cell lines, specifically designed to replicate patient samples in research and diagnostic settings. This high-quality FFPE standard is an essential workflow control for use in diagnostic laboratories and research and development (R&D) departments, supporting assay validation, FFPE diagnostic kit development, and extraction kit optimization. For details on additional gene mutations, refer to the comprehensive list on the product page.
The PIK3CA-E545G 50% AF FFPE Reference Standard curl is tailored for quality control in clinical genomics workflows and is particularly effective for the following applications:
- Validation of extraction protocols: Ensures reliable DNA and RNA isolation, which is crucial for downstream assays.
- Control in sequencing protocols: Suitable for Whole Genome Sequencing (WGS), Whole Exome Sequencing (WES), amplicon sequencing, and PCR workflows.
- Detection limit determination: Helps labs establish sensitivity limits for accurate detection of low-level mutations.
- Instrument calibration: Assists in calibrating and developing DNA processing workflows, including DNA fragmentation through acoustic shearing, enzymatic digestion, or sonication.
- NGS pipeline performance analysis: Supports accuracy and reliability across next-generation sequencing (NGS) workflows.
- Routine performance monitoring: Facilitates regular quality assessments for research applications (Research Use Only).
Product Overview
- Format: FFPE (Formalin Fixed Paraffin Embedded) standard
- Unit size: 1 curl per vial
- Expected DNA yield: At least 400 ng when using the Qiagen AllPrep DNA/RNA FFPE kit
- Expected RNA yield: At least 400 ng when using the Qiagen AllPrep DNA/RNA FFPE kit
- Curl thickness: Precisely 10 µm per curl
Mutation Profile and Testing
This reference standard includes 2,287 cancer-relevant mutations across 116 genes, offering a comprehensive tool for clinical and research workflows. The specific mutation PIK3CA-E545G (HGVS: c.1634A>G) has been verified using digital droplet PCR (ddPCR) to confirm a 50% allelic frequency, ensuring high accuracy and reproducibility. Orthogonal sequencing further confirms this mutation, adding an extra layer of reliability.
Key Attributes:
- Allelic frequency: PIK3CA-E545G at 50%, verified by ddPCR.
- Storage conditions: Recommended storage at 2-8°C.
- Shelf life: Stable for 24 months from the manufacturing date.
The PIK3CA-E545G 50% AF FFPE Reference Standard curl is a valuable tool for improving the consistency, reliability, and reproducibility of results in genetic testing, making it indispensable for clinical genomics and molecular diagnostics.
PIK3CA-E545G 50% AF FFPE Reference Standard curl
Datasheets and regulatory documentation
Everything your quality team needs for evaluation and procurement.
Frequently paired with this kit
Other assays and instruments lab teams add alongside the PIK3CA-E545G 50% AF FFPE Reference Standard curl.
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