PML-RARA Real-Time PCR kit

Specifications of the PML RARA Real-Time PCR Kit for Acute Progranulocytic Leukemia Research

The PML RARA Real-Time PCR Kit offers a comprehensive solution for the study and diagnosis of acute progranulocytic leukemia (APL). It has been designed to address multiple variables in a single assay, ensuring high levels of sensitivity and specificity.

Sensitivity and Limit of Quantification This kit features an MR4 detection threshold, set at 0.01% of PML-RARA, making it a valuable tool for the quantification of minimal residual disease (MRD). The Limit of Quantification (LOQ) is calibrated at 125 total copies for both PML-RARA and the endogenous GUS reference gene.

Dual-Target Analysis Simultaneous quantification of PML-RARA:bcr1 and endogenous GUS gene copies is a central feature of this kit. The ability to quantify both the target and a reference gene provides a reliable method for data validation in real-time assays.

Operational Efficiency Designed with workflow considerations, the kit requires a manual hands-on time of 45 minutes and a PCR program runtime of 1 hour and 15 minutes. This contributes to expedited data collection and interpretation.

Sample Integrity and Control The inclusion of an endogenous GUS gene control serves to validate both sample quality and integrity. The control is integrated into the kit for the facilitation of precise and reliable relative quantification metrics.

Instrument Compatibility The kit is compatible with FAM™ Real-time PCR cyclers, allowing for its integration into a range of existing lab setups without the need for additional investment in specialized equipment.

Sample Requirements and High-Throughput Capacity Optimized for use with peripheral blood-derived RNA samples, the kit requires 20µL of cDNA synthesized from 1µg of total RNA. The capacity to perform up to four parallel reactions per sample enhances the potential for high-throughput data acquisition.

Quality Standards The kit is manufactured under conditions that are compliant with ISO 9001 and ISO 14001 standards. These benchmarks ensure that you are working with materials that meet international quality and sustainability criteria.

In summary, the PML RARA Real-Time PCR Kit offers a robust, efficient, and precise method for the study of APL. Its design considerations make it a reliable choice for professionals in molecular biology and hematology research.

The kit contains the necessary reagents to perform 48 reactions. The reagents included in the kit are the following:

• PML-RARA Master Mix: Oligonucleotides and hydrolysis probes (FAMTM) specifically designed to detect the PML-RARA:bcr1 rearrangement.
• GUS Master Mix: Oligonucleotides and hydrolysis probes (FAMTM) specifically designed to detect the reference gene GUS.
• PML-RARA Standard: Plasmid at a concentration of 25 x 104 copies/µL of PML-RARA and GUS in a 1:1 ratio.