Cancer Profiling Kit
The Cancer Profiling Kit is a next-generation sequencing panel for the genetic characterisation of solid tumours. It covers 56 genes including hotspot regions and fusion genes, contains a 110-microsatellite panel for microsatellite instability testing, and analyses pharmacogenetic variants for chemotherapy dose adjustment. One kit covers 24 reactions. Research use only.
Quote-based, no public pricing. Add to your quote and our specialists put together a proposal with the right documentation, typically within 24 hours.
This kit in detail
- 56-gene panel for solid tumours
- Hotspot regions and fusion gene detection
- 110-microsatellite panel for microsatellite instability testing
- Pharmacogenetic variants for chemotherapy dose adjustment
- Variant detection in low-quality DNA
- 24 reactions per kit, with 24 unique 8-nucleotide indexes
The Cancer Profiling Kit analyses solid tumours by next-generation sequencing. Sequencing multiple genomic regions in one run gives a view of the tumour genotype that single-gene testing cannot provide.
Panel content
The panel covers 56 genes implicated in a range of cancers, and includes coverage of hotspot regions and of fusion genes.
Microsatellite instability
A 110-microsatellite panel is included for microsatellite instability testing, which is used to identify cases that may respond to immunotherapy.
Pharmacogenetics
The kit analyses pharmacogenetic variants, which provide optional guidance for chemotherapy dose adjustment and thereby for limiting adverse reactions.
Sensitivity
The assay is designed to detect variants in DNA of low quality as well.
Workflow
Library preparation uses Illumina adapters. The kit supplies 24 unique 8-nucleotide indexes in single-use strips, so 24 samples can be identified within one sequencing run.
Regulatory status
This kit is for research use only.
The kit contents the necessary reagents to perform 24 reactions. The reagents included in the kit are the following:
- Fragmentation Buffer: Buffer used for DNA fragmentation, prior to NGS libraries preparation.
- Fragmentation Enzyme: Enzyme used for DNA fragmentation, prior to NGS libraries
preparation. - Reagents Plate 1: Plate with all the reagents required to perform the DNA end repair, the
Illumina adapters linkage, and amplifications done during the library preparation protocol. - Beads and Buffers plate: Plate with magnetic particles and wash buffers, required for the DNA
capture and its purification during the library preparation protocol. - Index: Oligonucleotides with a unique 8 nucleotides identifier sequence compatible with the
illumina adapters required to identify each sample during library preparation and NGS
sequencing. The kit includes 24 different indexes, prepared in single-use strips. - Action ST Probes Strips 1: Biotinylated synthetic oligonucleotides complementary to the target
regions of the target genes. - Elution Buffer: Buffer used to elute the DNA.
Datasheets and regulatory documentation
Everything your quality team needs for evaluation and procurement.
Instructions for Use
MSDS Cancer Profiling Kit
Frequently paired with this kit
Other assays and instruments lab teams add alongside the Cancer Profiling Kit.
Add this kit to your quote
Tell us the platforms and throughput you’re planning for and our specialists will put together a clear proposal with the right documentation, no public pricing, just a considered answer.
