RAA Fluorescent Nucleic Acid Amplification

RAA Fluorescent Nucleic Acid Amplification

The RAA Fluorescent Nucleic Acid Amplification is developed for fast, sensitive nucleic acid amplification with real-time fluorescence detection. Designed for professional laboratory use, the kit amplifies DNA or RNA targets using recombinase-aided amplification (RAA), an isothermal technology that mimics natural cellular processes to synthesize double-stranded DNA at a constant temperature.

Operating at temperatures between approximately 37–42 °C, the system enables rapid nucleic acid amplification without the need for thermal cycling. When combined with a fluorescence probe, the reaction produces real-time fluorescence signals that allow continuous monitoring of amplification as it occurs. This approach enables highly sensitive detection while maintaining a simplified molecular workflow.

The kit includes lyophilized amplification tubes containing the enzyme mix, together with essential reaction components optimized for efficient amplification and fluorescence signal generation.

Kit Components

The RAA Fluorescent Nucleic Acid Amplification typically includes the following components:

• Lyophilized amplification tubes containing enzyme mix

• Buffer VI

• Magnesium acetate I

• Positive control material

• Forward primer for positive control

• Reverse primer for positive control

• Fluorescence probe for positive control

• Purified water

• Instruction manual

Supported Targets

The kit supports nucleic acid amplification of:

• DNA targets

• RNA targets (when using RT-RAA variants)

• Synthetic control templates

• Extracted nucleic acids from biological samples

Compatible Applications

Typical workflows include:

• Molecular assay development

• Rapid nucleic acid screening

• Research-based pathogen detection

• Validation of custom primer and probe sets

Simple and Streamlined Protocol

Real-time fluorescence amplification workflow

• Prepare mastermix containing Buffer VI, primers, fluorescence probe, and water

• Dispense mastermix into amplification tubes containing the lyophilized enzyme mix

• Add magnesium acetate and nucleic acid template

• Mix gently and briefly centrifuge the tubes

• Place reaction tubes in a real-time fluorescence detection instrument

• Incubate at approximately 39 °C and monitor amplification in real time

The amplification process typically generates detectable fluorescence signals within 15–30 minutes, allowing rapid nucleic acid detection for research and assay development workflows.