Respiratory Bacterial Pathogen Panel Kit

What is in the Respiratory Bacterial Panel?

The TRUPCR® Respiratory Bacterial Pathogen Panel Kit is a real-time PCR assay designed to detect 14 clinically significant respiratory bacterial pathogens from a single respiratory sample. The test is structured across four multiplexed tubes, each using four fluorophores (FAM, HEX/VIC, ROX/Texas Red, Cy5) to identify multiple targets simultaneously.

Bacterial Targets Included

Tube 1
Staphylococcus aureus, Streptococcus pneumoniae, Klebsiella pneumoniae, Mycoplasma pneumoniae

Tube 2
Salmonella spp., Streptococcus pyogenes, Bordetella spp., Chlamydia pneumoniae

Tube 3
Streptococcus agalactiae, Acinetobacter baumannii, Pseudomonas aeruginosa, Legionella pneumophila

Tube 4
Haemophilus influenzae (A–F), Moraxella catarrhalis and Endogenous Internal Control for sample adequacy and assay validation

This focused respiratory bacterial coverage supports precise diagnostics, rapid differentiation of common, atypical, and hospital-acquired pathogens, and improved clinical decision-making.

About the TRUPCR® Respiratory Bacterial Pathogen Panel Kit

The kit is built on high-performance multiplex real-time PCR with:

• Four pre-aliquoted multiplex tubes with validated primer/probe mixes
• Hot-start DNA polymerase for enhanced specificity
• Endogenous internal control for extraction and PCR quality checks
• Compatibility with major qPCR instruments (ABI 7500 Fast, QuantStudio™ 5, Bio-Rad® CFX96)

Each target is designed against conserved genomic regions to ensure broad strain coverage and minimize cross-reactivity.

Principle and Workflow

1. Sample Collection & Extraction
   Respiratory specimens such as BAL, tracheal aspirate, sputum, or nasopharyngeal aspirate in VTM are processed using standard total nucleic acid extraction methods.
2. Multiplex PCR Setup
   The extracted nucleic acid is added to four separate tubes, each pre-loaded with a target-specific multiplex master mix containing primers and probes for designated bacterial pathogens.
3. Real-Time Amplification
   The assay runs for 40 cycles on a compatible Real-Time PCR instrument, with fluorescence detection across four channels: FAM, HEX/VIC, ROX, and Cy5.
4. Result Interpretation
   The endogenous internal control confirms sample quality and assay validity. Positive controls generate expected Ct values, supporting reliable interpretation of detected respiratory bacterial targets.