Rift Valley Fever PCR Kit
The Rift Valley Fever Virus (RVFV) PCR Detection Kit is a high-performance real-time RT-qPCR assay designed for the qualitative detection of RVFV RNA. Utilizing advanced hydrolysis probe (TaqMan®) technology, the assay ensures highly sensitive and specific identification of this zoonotic arbovirus in veterinary and research samples. Targeting conserved regions of the RVFV genome, the kit enables reliable detection across circulating strains. An integrated internal control system verifies RNA quality and detects potential PCR inhibition, ensuring accurate and reproducible results. With optimized one-step RT-qPCR chemistry and compatibility with major real-time PCR platforms, this kit provides an efficient solution for outbreak monitoring and veterinary diagnostics.
Quote-based, no public pricing. Add to your quote and our specialists put together a proposal with the right documentation, typically within 24 hours.
This kit in detail
The Rift Valley Fever Virus (RVFV) PCR Detection Kit is a high-performance real-time RT-qPCR assay designed for the qualitative detection of RVFV RNA. Utilizing advanced hydrolysis probe (TaqMan®) technology, the assay ensures highly sensitive and specific identification of this zoonotic arbovirus in veterinary and research samples.
Targeting conserved regions of the RVFV genome, the kit enables reliable detection across circulating strains. An integrated internal control system verifies RNA quality and detects potential PCR inhibition, ensuring accurate and reproducible results.
With optimized one-step RT-qPCR chemistry and compatibility with major real-time PCR platforms, this kit provides an efficient solution for outbreak monitoring and veterinary diagnostics.
- High sensitivity for early RVFV RNA detection
- High specificity for accurate viral identification
- Dual-channel detection with internal control (FAM/HEX)
- Ready-to-use one-step RT-qPCR workflow
Qualitative RT-qPCR Detection of RVFV RNA
The RVFV PCR Detection Kit is an in-vitro real-time RT-qPCR assay developed for the qualitative identification of Rift Valley Fever Virus RNA. The assay utilizes hydrolysis probe technology to amplify and detect specific viral targets with high sensitivity and specificity.
The kit includes a ready-to-use master mix containing primers and probes for RVFV detection and an internal control, enabling simultaneous validation of sample quality and amplification efficiency.
Molecular Background
Rift Valley Fever Virus (RVFV) is a single-stranded RNA virus that primarily infects livestock such as cattle, sheep, and goats, but can also infect humans. The virus is mainly transmitted through mosquito vectors, although infection can also occur via contact with infected animal tissues or fluids.
In animals, RVFV infection can cause high mortality rates and widespread abortions, leading to significant economic losses. In humans, the disease typically presents as a mild febrile illness but can progress to severe complications such as encephalitis, haemorrhagic fever, or ocular disease.
Assay Performance & Utility
The assay is designed for rapid and reliable detection of RVFV RNA in veterinary and research samples. One-step RT-qPCR allows reverse transcription and amplification in a single reaction, improving workflow efficiency and reducing handling time.
Hydrolysis probe-based detection ensures high specificity, while optimized assay conditions provide robust performance even in low viral load samples. The internal control system supports confident interpretation of both positive and negative results.
Technical Specifications
- Assay Type: Qualitative Real-Time RT-qPCR
- Target: Rift Valley Fever Virus (RVFV) RNA
- Detection Channels: FAM (RVFV), HEX (Internal Control)
- Sensitivity: High analytical sensitivity (validated internally)
- Sample Types: Veterinary samples (e.g. blood, tissue, serum – validated in-house)
- Input Material: Extracted RNA
- Technology: One-step RT-qPCR with hydrolysis probe detection
- Compatible Instruments: ABI 7500, QuantStudio series, Bio-Rad CFX, Rotor-Gene Q, LightCycler 480, and equivalent systems
- qPCR Master Mix
- Positive control
- Nuclease-free water
Datasheets and regulatory documentation
Everything your quality team needs for evaluation and procurement.
Documentation available on request, contact us and we will send it over.
Frequently paired with this kit
Other assays and instruments lab teams add alongside the Rift Valley Fever PCR Kit.
iCassette - Mycobacterium Tuberculosis and Rifampicin resistance
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