X
- No products in the list
Two hundred million people are suffering from schistosomiasis in sub-topical areas. Patients become infected when bathing in contaminated fresh water. The disease is confirmed by microscopy when eggs are detected in stool or urine samples. However when the worm burden is light or during the invasive phase, few or no eggs are excreted. In such situations the serodiagnosis is a useful complement to parasitolgical tests. The diagnosis of human schistosomiasis using the ELISA kit is intended for travelers with frequent contacts with fresh water returning from endemic areas, for travel companions of confirmed cases, to post therapeutic controls and to control programs in endemic areas.
96 determinations of IgG antibodies using standard methodologies.
Wells sensitized with soluble antigens from Schistosoma mansoni.
8-wells polystyrene breakable strips: useful and economic format for laboratories with a small workload.
CE registration: H-CH/CA01/IVD/17983
The test detects both Schistosoma mansoni and Schistosoma haematobium infections.
A sensitivity of 94% was found with sera from patients with parasitologically proven Schistosoma mansoni and/or Schistosoma haematobium infections.
A specificity of 99% was found with 122 sera of blood donors.
A specificity of 94% was found with patients with other parasitc infections.
96 wells coated with soluble antigens from Schistosoma mansoni.
Dilution buffer (TBS-Tween), 50 ml
Washing solution, 50 ml
Enzyme buffer, 50 ml
Stop solution, 25 ml
Negative control serum (rabbit), 200 µl
Cut-off serum (rabbit), 200 µl
Positive control serum (rabbit), 200 µl
Protein A – AP conjugate, 300 µl
Alk. phosphatase substrate, 20 tab.
Multipipette resevoir 25ml, 1x
Frame for ELISA 8-wells holder, 1x
Instructions for use
Instructions for Use
References
Doenhoff, M.J., Wheeler, J.G., Tricker, K., Hamilton, J.V., Sturrock, R.F., Butterworth, A.E., Ouma, J.H., Mbugua, G.G., Kariuki, C. and Koech, D. (2003) The detection of antibodies against Schistosoma mansoni soluble egg antigens (SEA) and CEF6 in ELISA, before and after chemotherapy. Ann. Trop. Med. Parasitol. 97, 697-709.
Turner, P., Lalloo, K., Bligh, J., Amstrong, M., Whitty, C.J.M., Doenhoff, M.J. and Chiodini, P.L. (2004) Serological speciation of human schistosome infections by ELISA with a panel of three antigens. J. Clin. Pathol. 57, 1193-1196.
Sorgho, H., Bahgat, M., Poda, J., Song, W., Kristen, C., Doenhoff, M.J., Zongo, I., Ouedraogo, J. and Ruppel, A. (2005) Serodiagnosis of Schistosoma mansoni infections in endemic area of Burkina Faso: performance of several immunological tests with different parasite antigens. Acta Tropica. 93, 169-180.