SwiftX™ Parabact Extraction

SwiftX™ ParaBact Extraction Kit

The SwiftX™ ParaBact is developed for rapid and sensitive DNA extraction from bacteria and eukaryotic cells across a wide range of biological specimens. Designed for professional laboratory use, the kit isolates high-quality DNA from bacteria, parasitic protozoa, helminth eggs, fungi, and human or animal cells using a magnetic cell capture workflow combined with heat-assisted lysis and reverse purification.

Its flexible protocol options support both direct extraction from solid specimens and enrichment-based workflows for liquid samples. By enabling species-independent capture of cells prior to lysis, SwiftX™ ParaBact improves sensitivity and enables efficient DNA extraction even from low-abundance microorganisms. The workflow produces amplification-ready DNA suitable for PCR, isothermal amplification technologies, and sequencing applications.

The kit includes Buffer BPE, Buffer BPL, Buffer AD, and paramagnetic Beads A, each optimized to support efficient cell capture, lysis, and purification. Buffer BPE stabilizes cells and promotes binding to the magnetic particles during the capture step. Buffer BPL enables efficient lysis of bacterial, parasitic, fungal, and mammalian cells, particularly when combined with heat incubation. Buffer AD neutralizes the lysate and ensures compatibility with downstream molecular assays. The paramagnetic Beads A enable both species-independent cell concentration and removal of debris during the reverse purification step.

SwiftX™ ParaBact supports DNA extraction from:

• Bacteria (Gram-positive and Gram-negative)
• Parasitic protozoa and helminth eggs
• Fungi
• Human and animal cells

Compatible sample types include:

Solid samples: oral, nasal, rectal or lesion swabs, pre-concentrated cells
Liquid samples (up to 1 mL): urine, saliva, sputum, cerebrospinal fluid, tissue homogenates, blood, cell cultures, wastewater, transport media, and liquid-based cytology samples

Simple and Streamlined Protocol

Protocol 1 | Direct extraction workflow for solid samples

1. add sample to Buffer BP
2. add Beads A and mix
3. incubate at 95°C for 5 to 15 minutes
4. add Buffer AD to neutralize lysate
5. place tube in magnetic rack
6. use supernatant for downstream application

Protocol 2 | Workflow for liquid samples with cell capture

1. prepare capture mix of Buffer BPE and Beads A
2. add liquid sample and incubate 3 minutes at ambient temperature
3. place tube in magnetic rack and discard supernatant
4. resuspend captured cells in Buffer BPL
5. incubate at 95°C for 5 to 15 minutes
6. add Buffer AD
7. place tube in magnetic rack
8. use supernatant for downstream application

Protocol 3 | Workflow for complex liquid samples

1. perform cell capture with Buffer BPE and Beads A
2. separate beads magnetically and discard supernatant
3. wash captured cells once with Buffer BPE
4. resuspend beads in Buffer BPL
5. incubate at 95°C for 5 to 15 minutes
6. add Buffer AD
7. place tube in magnetic rack
8. use supernatant for downstream application