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The SwiftX™ Sepsis extraction Kit enables rapid purification of bacterial, fungal, and host DNA from whole blood, supporting molecular research in sepsis and bloodstream infections. Optimized for venous whole blood anticoagulated with citrate or EDTA, the kit ensures efficient recovery of DNA from up to 10 mL input volume while maintaining workflow simplicity.
The technology is based on magnetic bead–mediated cell capture. Buffer FBC stabilizes biological cells and promotes preferential binding of microbial cells and leukocytes to Beads A. Following magnetic separation, wash steps with Buffer FBW remove hemoglobin, plasma components, and PCR inhibitors. Subsequent lysis using Buffer FBL in combination with Proteinase K and a two-step heat incubation ensures efficient DNA release.
This approach significantly reduces inhibitor carryover compared to conventional extraction methods, resulting in DNA eluates suitable for sensitive downstream amplification. The purified DNA can be applied directly in qPCR, isothermal amplification, and other nucleic acid–based analytical techniques without additional cleanup procedures.
SwiftX™ Sepsis extraction kit is designed for research use only and provides laboratories with a robust solution for rapid and reproducible DNA preparation from complex blood samples.
For Research Use Only (RUO)
The SwiftX™ Sepsis extraction Kit provides rapid and sensitive extraction of bacterial, fungal, and leukocyte DNA from venous whole blood, offering a streamlined workflow for molecular research in sepsis and bloodstream infections. Designed for manual purification from citrate- or EDTA-anticoagulated blood, the method supports input volumes from 400 µL up to 10 mL, allowing laboratories to tailor sensitivity according to clinical research needs.
The extraction chemistry is based on a magnetic bead–driven cell capture principle rather than direct total lysis of blood. Buffer FBC stabilizes intact biological cells and promotes selective binding of microbial and white blood cells to paramagnetic Beads A. This targeted capture enables effective separation of cells from plasma components and inhibitory substances through magnetic isolation.
After capture, Buffer FBW is applied in dedicated wash steps to remove residual contaminants, hemoglobin, and potential PCR inhibitors. Efficient and complete supernatant removal during these steps is critical to achieving optimal downstream performance. The subsequent lysis step is performed using Buffer FBL in combination with Proteinase K, followed by a two-stage heat incubation at 65 °C and 95 °C. This ensures thorough cell disruption and release of nucleic acids while maintaining workflow consistency.
The resulting DNA eluate can be directly used for real-time quantitative PCR, isothermal amplification, and other nucleic acid–based analytical techniques. Typical DNA concentrations from 10 mL whole blood are approximately 200 ng/µL, predominantly reflecting total DNA content. Importantly, total DNA concentration does not necessarily correlate with microbial DNA levels, making direct amplification-based detection the recommended analytical approach.
SwiftX™ Sepsis extraction kit reagents are stored at 2–8 °C and remain stable until the indicated expiry date. After first use, the kit maintains performance for up to 3 months when stored properly. The method is optimized for use with high-quality magnetic stands featuring strong neodymium magnets to ensure efficient particle separation and reproducible results.
Intended for research use only, SwiftX™ Sepsis extraction kit enables laboratories and assay developers to accelerate sepsis-related molecular workflows while ensuring reliable DNA recovery from one of the most complex biological sample types—whole blood.
For Research Use Only (RUO)