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Pre-analytical Products
TruPCR Distributed by Goffin Diagnostics

Tissue DNA Extraction Kit – TRUPCR®

Tissue DNA extraction is streamlined and highly efficient with the TRUPCR® Tissue DNA Extraction Kit, designed for rapid isolation of high-purity DNA from animal tissue, cells, bacteria, and blood. The silica membrane–based system utilizes a proteinase K and SDS lysis protocol followed by specific binding, washing, and elution steps. With a flexible elution range and robust contaminant removal, this kit delivers consistent yields of PCR-ready DNA from a wide range of sample types. The protocol eliminates hazardous reagents and supports various molecular applications, including PCR and NGS.

Silica Membrane CE-IVD TruPCR 1 Unit (48 Reactions), 1 Unit (96 Reactions)
Technique
Silica Membrane
Brand
TruPCR
Packaging
1 Unit (48 Reactions), 1 Unit (96 Reactions)
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Product details

This kit in detail

Tissue DNA extraction is streamlined and highly efficient with the TRUPCR® Tissue DNA Extraction Kit, designed for rapid isolation of high-purity DNA from animal tissue, cells, bacteria, and blood. The silica membrane–based system utilizes a proteinase K and SDS lysis protocol followed by specific binding, washing, and elution steps. With a flexible elution range and robust contaminant removal, this kit delivers consistent yields of PCR-ready DNA from a wide range of sample types. The protocol eliminates hazardous reagents and supports various molecular applications, including PCR and NGS.

  • Fast and flexible tissue DNA extraction from multiple sample types
  • Silica membrane–based purification for high yield and purity
  • No phenol-chloroform or hazardous chemicals
  • Compatible with PCR, qPCR, and downstream molecular workflows

What is Tissue DNA Extraction?

Tissue DNA extraction is the process of isolating high-quality DNA from biological tissues and other complex samples. This is a critical first step in molecular diagnostics, genotyping, and pathogen detection. The TRUPCR® Tissue DNA Extraction Kit offers a reliable, scalable method for isolating both genomic and microbial DNA from diverse sample sources including tissue biopsies, cultured cells, and body fluids.

Importance of High-Quality DNA in Clinical Applications

High-quality DNA is the foundation of accurate PCR, sequencing, and diagnostic workflows. Inconsistent yield, contamination, or inhibitors can compromise test results. The TRUPCR® Tissue DNA Extraction Kit provides the purity, yield, and reproducibility necessary for high-throughput laboratories and clinical diagnostics.

About the TRUPCR® Tissue DNA Extraction Kit

This kit follows a proven 4-step method:

  1. Enzymatic Lysis – A proteinase K/SDS lysis buffer breaks down cell membranes and releases DNA

  2. Binding – DNA binds to a silica membrane under chaotropic conditions with ethanol

  3. Washing – Efficient wash steps remove proteins, salts, and PCR inhibitors

  4. Elution – Purified DNA is eluted in a user-defined volume (20–100 µL)

The process is optimized for recovery of DNA longer than 30 kb and yields highly pure DNA suitable for PCR, real-time PCR, and NGS applications.

Principle and Workflow

  • Technology: Silica column

  • Sample Types: Animal tissue, bacterial cultures, blood, body fluids

  • Applications: PCR, qPCR, NGS, sequencing

  • Elution Volume: 20–100 µL

  • Processing: Manual (centrifugation), hands-on time ≤ 1 hour

  • Yield: High consistency and reproducibility from minimal sample input

All buffers are optimized to ensure complete lysis, high DNA binding, and efficient removal of inhibitors-ensuring DNA of the highest integrity.

Clinical and Research Applications

The TRUPCR® Tissue DNA Extraction Kit is a versatile solution for:

  • Pathogen detection from infected tissues

  • Genomic studies using biopsies or animal models

  • Cancer research via DNA from tumor tissues

  • Molecular diagnostics for both host and microbial targets

  • NGS library prep requiring high-purity DNA

Whether you’re processing clinical biopsies or lab-cultured samples, this kit ensures reproducible and inhibitor-free DNA extraction.

Documentation

Datasheets and regulatory documentation

Everything your quality team needs for evaluation and procurement.

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