Ultra Deep Microbiome Prep 10 Kit
Ultra-Deep Microbiome Prep10 isolates bacterial and fungal DNA from body fluids of 1 to 10 ml and from tissue pieces of about 0.5 by 0.5 cm, after depleting at least 95 per cent of the host DNA. Research use only. The chemistry is the same as in Ultra-Deep Microbiome Prep; the difference is the sample volume. The larger volume raises the number of microbial cells that reach the column, which matters when the load is low.
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This kit in detail
- Sample volume 1 to 10 ml, with concentration of the microbial cells
- Removal of at least 95 per cent of host DNA
- Broad lysis: over 200 bacterial genera and 65 fungal genera
- MolYsis chemistry with MolDNase B and BugLysis
- Requires a high-speed centrifuge for 50 ml tubes at 9,500 x g
- 25 or 50 extractions per kit, REF G-030-025 and G-030-050
Ultra-Deep Microbiome Prep10 isolates bacterial and fungal DNA from larger sample volumes, 1 to 10 ml, with the same host depletion as the 1 ml version.
Principle
A chaotropic buffer lyses the human cells in the sample while microbial cells stay intact. The human DNA released, including DNA from dead cells, is degraded by the chaotrope-resistant DNase MolDNase B. The microbial cells are then collected and their cell walls degraded with BugLysis, followed by Proteinase K digestion and spin-column purification of the microbial DNA. The microbial cells are concentrated by centrifugation before lysis, which is what allows the larger input volume to be used. The manufacturer states removal of at least 95 per cent of host DNA, and lysis has been shown on over 200 bacterial genera and 65 fungal genera.
Sample material and equipment
Body fluids: ascites, bronchoalveolar lavage, blood, cerebrospinal fluid, pleural fluid, pus and synovial fluid, 1 to 10 ml. Tissue pieces of about 0.5 by 0.5 cm. Besides a thermomixer and a bench-top microcentrifuge, a high-speed centrifuge for 50 ml tubes at 9,500 x g is required. For volumes up to 1 ml the manufacturer offers Ultra-Deep Microbiome Prep.
What is and is not supplied
Buffers and consumables at room temperature, enzymes frozen, and 50 ml centrifuge tubes as a third component. No controls are supplied; an internal PCR control is available separately as Ultra-Deep Microbiome Ampli IC (G-130-042). Shelf life is 24 months.
Regulatory status
This kit is for research use only and not for use in diagnostic procedures.
- Lysis Buffer: A unique buffer designed to efficiently lyse microbial cells and release DNA.
- Beads: A specially formulated mixture of beads that are used to mechanically disrupt microbial cells and improve DNA recovery.
- Wash Buffer: A buffer that is used to wash the beads and DNA during the purification process, removing contaminants and ensuring high purity.
- Elution Buffer: A buffer that is used to elute the purified DNA from the beads.
- Microcentrifuge Tubes: Tubes that are used to hold the samples during the lysis, purification, and elution steps.
- User Manual: A comprehensive guide that provides step-by-step instructions on how to use the kit and obtain high-quality DNA from microbiome samples.
All of these components are included in the Ultra Deep Microbiome Prep 10 kit, making it a complete solution for DNA extraction from microbiome samples.
Datasheets and regulatory documentation
Everything your quality team needs for evaluation and procurement.
Instructions for Use
Frequently paired with this kit
Other assays and instruments lab teams add alongside the Ultra Deep Microbiome Prep 10 Kit.
Add this kit to your quote
Tell us the platforms and throughput you’re planning for and our specialists will put together a clear proposal with the right documentation, no public pricing, just a considered answer.
