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The TRUPCR® VRE Detection Kit is a multiplex real-time PCR assay designed for the qualitative detection of Enterococcus faecalis and Enterococcus faecium, as well as the detection and differentiation of vancomycin resistance genes vanA and vanB. The assay uses a single-tube reaction format incorporating TaqMan® hydrolysis probe chemistry to provide target-specific amplification with high analytical sensitivity and specificity.
An endogenous internal control is included in each reaction to verify sample adequacy and monitor for PCR inhibition, ensuring interpretability of negative results. The assay supports DNA extracted from rectal swabs, urine specimens, or cultured isolates.
Reagents and controls are supplied ready-to-use, and the kit is compatible with widely used real-time PCR platforms. With validated sensitivity and specificity and the ability to detect both species and resistance markers in a multiplex format, the TRUPCR® VRE Detection Kit provides a standardized workflow for clinical laboratories performing molecular surveillance or diagnostic testing for vancomycin-resistant Enterococcus.
The TRUPCR® VRE Detection Kit is an in vitro real-time PCR assay intended for the qualitative detection of Enterococcus faecalis and Enterococcus faecium, and for identifying vancomycin resistance mediated by the vanA and vanB genes. The assay consolidates species identification and resistance detection into a single reaction, enabling efficient analysis of clinical samples or cultured isolates. The workflow is suitable for routine molecular testing in clinical microbiology laboratories.
Enterococcus faecalis and E. faecium are Gram-positive organisms associated with a variety of healthcare-associated infections, including urinary tract infections, bacteremia, and wound infections. Vancomycin-resistant Enterococcus (VRE) represents a significant clinical concern due to reduced therapeutic options, with resistance primarily mediated by the vanA and vanB gene clusters. Molecular identification of these genes supports detection of vancomycin resistance mechanisms but does not replace phenotypic susceptibility testing. Results should be interpreted alongside clinical and microbiological findings.
The assay uses fluorescent hydrolysis probes to target conserved regions of Enterococcus spp. and the vanA/vanB resistance genes. The single-tube multiplex format employs four distinct fluorophores to differentiate species signals from resistance markers. An endogenous internal control is included to assess sample integrity and detect potential PCR inhibition.
All components are supplied ready-to-use. The kit includes hot-start DNA polymerase to minimize non-specific amplification and uracil-DNA glycosylase (UNG) to reduce carryover contamination risk.
Extracted genomic DNA from urine, rectal swabs, or cultured isolates is added to the reaction mix. During amplification, species- and resistance-specific probes hybridize to target sequences. Probe cleavage during polymerase extension separates the fluorophore from the quencher, generating a fluorescence signal proportional to the amount of amplified target DNA.
The assay monitors fluorescence in each cycle, allowing determination of Ct values for species- and resistance-specific targets as well as the internal control. The internal control must amplify within the expected range to confirm that negative results are valid. The workflow includes DNA extraction, reaction setup, real-time amplification, and interpretation of fluorescence data.
The TRUPCR® VRE Detection Kit is intended for qualitative detection of VRE in clinical specimens and cultured isolates. Applications include screening, infection control surveillance, and diagnostic evaluation of suspected Enterococcus infections. While the assay detects vanA and vanB, a negative result does not exclude resistance mediated by other mechanisms. All results should be evaluated in combination with phenotypic testing and additional laboratory and clinical information.
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