Liquid Biopsy OncoKit
The Imegen Liquid Biopsy OncoKit is an NGS panel for circulating tumour DNA from patients with lung cancer, with automated library preparation on the Agilent Magnis system. Research use only. The panel covers the whole exonic regions of 13 genes, hotspot regions in NTRK1 and NTRK3, intronic coverage for 8 fusion genes, and 500 SNPs for genome-wide copy number analysis. The limit of detection for point variants and small insertions and deletions is 1 per cent.
Quote-based, no public pricing. Add to your quote and our specialists put together a proposal with the right documentation, typically within 24 hours.
This kit in detail
- Whole exonic regions of 13 genes, including EGFR, KRAS, BRAF, ALK and TP53
- Hotspots in NTRK1 (4 variants) and NTRK3 (2 variants)
- 8 fusion genes with intronic coverage, plus 500 SNPs for CNV analysis
- Limit of detection 1 per cent for point variants, insertions and deletions
- ctDNA from peripheral blood or pleural fluid, 10 to 50 ng
- 24 libraries per kit, REF IMG-415
The Imegen Liquid Biopsy OncoKit sequences circulating tumour DNA from patients with lung cancer, so that the tumour genotype can be determined without a tissue biopsy.
What the panel covers
The whole exonic regions of 13 genes: ALK, BRAF, EGFR, ERBB2, KIT, KRAS, MAP2K1, MET, NRAS, PIK3CA, RB1, ROS1 and TP53. Hotspot regions in NTRK1, four variants, and NTRK3, two variants. Intronic coverage for 8 fusion genes: ALK, EGFR, ETV6-NTRK3, NRG1, NTRK1, NTRK2, RET and ROS1. And 500 SNPs distributed across the genome for copy number analysis.
Sensitivity
The limit of detection for structural variants, point variants and small insertions and deletions is 1 per cent. For copy number gains the limit is 2.9 total copies, which corresponds to 4 copies at 50 per cent tumour content. The manufacturer reports sensitivity and specificity above 99 per cent, a mean coverage of 7300x and 2500x after UMI collapsing, with 96.4 per cent of bases at 100x.
Method and workflow
Library preparation is automated on the Agilent Magnis NGS Prep System and uses unique molecular identifiers, which is what allows a true variant to be distinguished from a sequencing artefact at 1 per cent. The input is 10 to 50 ng of ctDNA from peripheral blood or pleural fluid. Sequencing runs on an Illumina instrument, with the NextSeq recommended, and analysis in the Data Genomics platform. The kit is delivered as four boxes with the sample strips, reagent plates, capture probes and consumables for 24 libraries, in three runs of 8.
Regulatory status
This kit is for research use only.
The kit is equipped with reagents for 24 reactions, including:
- Reagents Plate1: Plate with all the reagents required to perform the DNA end repair, the
Illumina adapters linkage, and amplifications done during the library preparation protocol. - Beads and Buffers plate: Plate with magnetic particles and wash buffers, required for the DNA
capture and its purification during the library preparation protocol. - Index: Oligonucleotides with a unique 8 nucleotides identifier sequence compatible with the
illumina adapters required to identify each sample during library preparation and NGS
sequencing. The kit includes 24 different indexes, prepared in single-use strips. - Targeted Liquid Biopsy Probes Strip: Biotinylated synthetic oligonucleotides complementary
to the target regions of the target genes. - Elution Buffer: Buffer used to elute the DNA
Datasheets and regulatory documentation
Everything your quality team needs for evaluation and procurement.
Instructions for Use
MSDS Liquid Biopsy OncoKit
Frequently paired with this kit
Other assays and instruments lab teams add alongside the Liquid Biopsy OncoKit.
Add this kit to your quote
Tell us the platforms and throughput you’re planning for and our specialists will put together a clear proposal with the right documentation, no public pricing, just a considered answer.
