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Anisakidosis is caused by some sea fish nematodes of the family Anisakidae. Humans can be infected by eating raw or undercooked contaminated fish meat. Most infected people do not show any symptoms. However, in some cases, symptoms appear at the gastric stage (epigastric pain, nausea and vomiting), intestinal stage (abdominal pain, nausea, vomiting, and diarrhea) or at the allergic stage (hives, pruritus, angioedema and bronchospasm). Diagnosis is based on signs and symptoms, but, in many cases it is not conclusive for the diagnosis. Serological assays has an important place on the final diagnosis of the disease. Our ELISA kit have shown appropriate performances for the serological screening of both digestive and allergic anisakidosis.
96 determinations of IgG antibodies using standard methodologies.
Wells sensitized with E/S antigens from cultivated larvae of Anisakidae.
8-wells polystyrene breakable strips: useful and economic format for laboratories with a small workload.
CE registration: CH-202201-0009
The test detects IgG antibodies against parasites of Anisakidae family in human serum.
A sensitivity of 97% was found with 38 sera from patients suffering from digestive and/or allergic anisakidosis.
A specificity of 97%was found with 180 sera of blood donors (Swiss).
A specificity of 96%was found with 98 sera from patients of an infectiology unit (Swiss).
A specificity of 86% was found with 43 sera from anisakidosis suspected patients but where this disease had been certainly ruled out.
A specificity of 81% was found with 47 sera of patients with other parasitic infections.
96 wells coated with E/S antigens from cultivated larvae of Anisakidae.
Dilution buffer (TBS-Tween), 50 ml
Washing solution, 50 ml
Enzyme buffer, 50 ml
Stop solution, 25 ml
Negative control serum (rabbit), 200 µl
Cut-off serum (rabbit), 200 µl
Positive control serum (rabbit), 200 µl
Protein A – AP conjugate, 300 µl
Alk. phosphatase substrate, 20 tab.
Multipipette resevoir 25ml, 1x
Frame for ELISA 8-wells holder, 1x
Instructions for use
Instructions for Use
References
Mazzucco, M., Raia, D.D., Marotta, C., Costa, A., Ferrantelli, V., Vitale, F. and Casuccio, A. (2018) Validation of an Anisakis sensitization in different population groups and public health impact: A systematic review. Plos one 13.
Kochanowski, M., Gonzalez-Munoz, M., Gomez-Morales, M.A., Gottstein, B., Dabrowska, J., Rozycki, M., Cencek, T., Muller, N. and Boubaker, G. (2019) Comparative analysis of excretory-secretory antigens of Anisakis simplex, Pseudoterranova decipiens and Contracaecum osculatum regarding their applicability for specific serodiagnosis of human anisakidosis based on IgG ELISA. Experimental Parasitology 197, 9-15.