BLOT-LINE CMV IgG

Description

• Antigen used: recombinant antigens of Cytomegalovirus (p150, p65, p52, p28).
• Recombinant antigens are transferred to the nitrocellulose membrane (i.e. to the respective BLOT ‐ LINE (BL) strips).
• If specific antibodies are present in the sample, they will bind to the respective antigens.
• The complex is labelled with Conjugate and detected through a colour reaction with substrate (BCIP/NBT).
• The kit enables 20 tests.
• Short incubation periods, total assay time: approximately 1.5 h.
• High sensitivity and specificity.
• All reagents supplied are ready to use.
• The kit may be used sequentially for smaller batches of samples (reagents are provided in sufficient quantities).
• BL strips equipped with conjugate control band and a control band indicating kits functionality and sensitivity.
• Colour identification in compliance with antibody classes.
• Identical (i.e. interchangeable) reagents (except for Conjugate) in all BLOT-LINE kits.
• Evaluation of results – the intensity of the bands can be evaluated visually or by means of software (Immunoblot Software).

Application

• Diagnostics of CMV.
• Confirmatory test to ELISA.

Brief assay procedure

1. Dilute serum/plasma samples (1:51).
2. Pipette diluted samples and controls into the channels of the tray and insert the BL strips.
3. Incubate for 30 min at room temperature using a shaking apparatus.
4. Wash 3 times for 5 min.
5. Add Conjugate.
6. Incubate for 30 min at room temperature using a shaking apparatus.
7. Wash 3 times for 5 min.
8. Add substrate (BCIP/NBT).
9. Incubate for 15 min at room temperature using a shaking apparatus.
10. Wash 2 times for 5 min in distilled water.
11. Dry the strips and evaluate results using the validation strip enclosed or using Immunoblot Software.

Specifications

• EAN Code: 8595635309533
• Catalog number: CMGL20
• Package size: 20 tests
• Regulatory status: IVD CE2265
• Storage: 2-8 °C
• Producer: TestLine Clinical Diagnostics s.r.o.