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BLOT-LINE Toxoplasma IgM
Infectious Serology · Toxoplasma Blot
TestLine Distributed by Goffin Diagnostics

BLOT-LINE Toxoplasma IgM

The immunoblot assay is intended for the diagnosis of Toxoplasma gondii infection using IgM antibodies in human serum or plasma in the general population. The qualitative and semi-quantitative manual assay is designed for professional use in a laboratory.

Immunoblot CE-IVD TestLine 1 Package (20 Tests)
Technique
Immunoblot
Brand
TestLine
Packaging
1 Package (20 Tests)
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Quote-based, no public pricing. Add to your quote and our specialists put together a proposal with the right documentation, typically within 24 hours.

Product details

This kit in detail

Description

  • Antigen used: recombinant antigens of Toxoplasma gondii (SAG1, MIC3, GRA1, GRA7, GRA8, SAG2).
  • Recombinant antigens are transferred to the nitrocellulose membrane (i.e. to the respective BLOT ‐ LINE (BL) strips).
  • If specific antibodies are present in the sample, they will bind to the respective antigens.
  • The complex is labelled with Conjugate and detected through a colour reaction with substrate (BCIP/NBT).
  • The kit enables 20 tests.
  • Short incubation periods, total assay time: approximately 1.5 h.
  • High sensitivity and specificity.
  • All reagents supplied are ready to use.
  • The kit may be used sequentially for smaller batches of samples (reagents are provided in sufficient quantities).
  • BL strips equipped with conjugate control band and a control band indicating kits functionality and sensitivity.
  • Colour identification in compliance with antibody classes.
  • Identical (i.e. interchangeable) reagents (except for Conjugate) in all BLOT-LINE kits.
  • Evaluation of results – the intensity of the bands can be evaluated visually or by means of software (Immunoblot Software).

Application

  • Diagnostics of infection with Toxoplasma gondii.
  • Confirmatory test to ELISA.

Brief assay procedure

  1. Dilute serum/plasma samples (1:51).
  2. Pipette diluted samples and controls into the channels of the tray and insert the BL strips.
  3. Incubate for 30 min at room temperature using a shaking apparatus.
  4. Wash 3 times for 5 min.
  5. Add Conjugate.
  6. Incubate for 30 min at room temperature using a shaking apparatus.
  7. Wash 3 times for 5 min.
  8. Add substrate (BCIP/NBT).
  9. Incubate for 15 min at room temperature using a shaking apparatus.
  10. Wash 2 times for 5 min in distilled water.
  11. Dry the strips and evaluate results using the validation strip enclosed or using Immunoblot Software.

Specifications

  • EAN Code: 8595635309571
  • Catalog number: TgML20
  • Package size: 20 tests
  • Regulatory status: IVD CE2265
  • Storage: 2-8 °C
  • Producer: TestLine Clinical Diagnostics s.r.o.
Documentation

Datasheets and regulatory documentation

Everything your quality team needs for evaluation and procurement.

PDF IVD_ES CERTIFICATE QS-014/A 3ECPDF · download PDF LEAFLET_Toxoplasma_ENPDF · download PDF IVDD_EC_CERTIFICATESPDF · download PDF IVDD_Extension of the certificatesPDF · download PDF LEAFLET_IMMUNOBLOT_full version_ENPDF · download
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