Chimerism Multiplex Plus I Kit
The Imegen Quimera Screening Multiplex Plus kit selects informative markers for the follow-up of patients after haematopoietic stem cell transplantation. It analyses 16 insertion and deletion polymorphisms in 8 multiplex real-time PCR reactions, two markers per tube. CE marked for in vitro diagnostic use. Together with the SRY and RhD markers the panel reaches a cumulative informativity of 99.1 per cent, and 99.98 per cent when it is combined with the Screening Multiplex Plus II panel. One kit covers 10 genomic DNA samples, which is 5 recipient and donor cases.
Quote-based, no public pricing. Add to your quote and our specialists put together a proposal with the right documentation, typically within 24 hours.
This kit in detail
- 16 insertion and deletion polymorphisms in 8 multiplex reactions
- Cumulative informativity 99.1 per cent, with SRY and RhD
- 99.98 per cent combined with Screening Multiplex Plus II
- Genomic DNA from peripheral blood, 450 ng of recipient and of donor
- 7500 Fast, StepOne Plus or LightCycler 480
- 10 DNA samples or 5 cases per kit, REF IMG-116-26
The Imegen Quimera Screening Multiplex Plus kit is the screening step in chimerism monitoring after an allogeneic stem cell transplant: it establishes which markers distinguish recipient from donor, so that one informative marker can then be followed quantitatively.
Markers
The panel contains 16 insertion and deletion polymorphisms, distributed over 8 tubes with two markers per tube: 3I, 4I, 5I, 6I, 7I, 10I, 11I, 12D, 23I, 24I, 27D, 28I, 29D, 30D, 31I and 32I. With the SRY and RhD markers included, the cumulative informativity is 99.1 per cent. Combining the panel with Screening Multiplex Plus II raises that to 99.98 per cent.
Method and sample
Detection is by real-time PCR with specific oligonucleotides and fluorescent hydrolysis probes. The limit of detection is 0.01 per cent. The material required is genomic DNA, mainly from peripheral blood, 450 ng of the recipient sample taken before transplantation and 450 ng of the donor sample. The kit consists of one strip of 8 tubes with freeze-dried master mix, rehydrated with 33 µl of water per tube, and each tube provides 10 reactions. The assay runs on the 7500 Fast or StepOne Plus (Thermo Fisher) or on the LightCycler 480 (Roche), with follow-up in the Imegen Quimera software.
Regulatory status
This kit is CE marked for in vitro diagnostic use.
The kit contians the necessary reagents to perform 10 reactions. The reagents included in the kit are the following:
- Specific Master-Mix: Specific oligonucleotides and labelled probes
Datasheets and regulatory documentation
Everything your quality team needs for evaluation and procurement.
Instructions for Use
MSDS Chimerism Multiplex Plus I Kit
Frequently paired with this kit
Other assays and instruments lab teams add alongside the Chimerism Multiplex Plus I Kit.
Add this kit to your quote
Tell us the platforms and throughput you’re planning for and our specialists will put together a clear proposal with the right documentation, no public pricing, just a considered answer.
