Chimerism dPCR Kit
The Imegen Quimera dPCR kit quantifies haematopoietic chimerism after transplantation by digital PCR. One informative marker, selected beforehand with the screening kits, is quantified against the reference gene beta-globin. CE marked for in vitro diagnostic use. The panel of available markers covers SRY, RhD and 31 insertion and deletion polymorphisms. The limit of detection is 0.01 per cent and the limit of quantification 0.05 per cent, which is the sensitivity that makes early detection of relapse or of graft loss possible.
Quote-based, no public pricing. Add to your quote and our specialists put together a proposal with the right documentation, typically within 24 hours.
This kit in detail
- Digital PCR quantification of one informative marker
- 33 markers available: SRY, RhD and 31 INDEL polymorphisms
- Reference gene beta-globin, detected with VIC alongside FAM for the marker
- Limit of detection 0.01 per cent, limit of quantification 0.05 per cent
- QuantStudio 3D, QuantStudio Absolute Q or Bio-Rad QX200
- 12 reactions per kit, REF IMG-116
The Imegen Quimera dPCR kit quantifies the proportion of recipient and donor cells after an allogeneic stem cell transplant. Digital PCR divides the sample into 20,000 partitions, which allows absolute quantification without a calibration curve.
Markers
One marker is quantified per reaction, selected in advance with the screening kits IMG-116-26 and IMG-116-25. Available are SRY, RhD and 31 insertion and deletion polymorphisms from the Q116 series. The master mix contains the oligonucleotides for the marker and for the reference gene beta-globin, with FAM for the marker and VIC for beta-globin.
Sensitivity
The limit of detection is 0.01 per cent and the limit of quantification 0.05 per cent. That sensitivity is what allows a rising recipient fraction to be seen before it becomes clinically apparent.
Method and sample
The material required is genomic DNA from peripheral blood, bone marrow or a separated cell subtype. The DNA input is 75 ng on Bio-Rad and 150 ng on Thermo Fisher platforms. The assay runs on the QuantStudio 3D, the QuantStudio Absolute Q or the Bio-Rad QX200 droplet system, with analysis in the corresponding platform software and follow-up in the Imegen Quimera software. Water instead of DNA serves as the negative control; beta-globin acts as the reference and internal check.
Regulatory status
This kit is CE marked for in vitro diagnostic use.
Specific Master-Mix X: This includes specific oligonucleotides and labeled probes.
Note: In the “X” in “Specific Master-Mix X”, it can refer to any of the listed markers, which range from RhD, Q116-6I, Q116-3I, and so on, as mentioned in the provided material safety data sheet.
Datasheets and regulatory documentation
Everything your quality team needs for evaluation and procurement.
Instructions for Use
Imegen® Quimera dPCR
Frequently paired with this kit
Other assays and instruments lab teams add alongside the Chimerism dPCR Kit.
Add this kit to your quote
Tell us the platforms and throughput you’re planning for and our specialists will put together a clear proposal with the right documentation, no public pricing, just a considered answer.
