EUROArray SwiftX-traction

EUROArray SwiftX-traction

EUROArray SwiftX-traction is developed for fast and reliable DNA extraction from a broad range of clinical specimen types. Designed for professional laboratory use, the kit isolates high-quality DNA from fungi, bacteria, parasites, viruses, and human epithelial cells using a workflow that combines targeted cell capture with rapid heat-lysis and magnetic particle technology. This approach enables efficient DNA purification while minimizing manual handling steps and processing time.

The kit includes Buffer EN, Buffer EAL, Proteinase K, and paramagnetic Beads A, each optimized for a specific function in the extraction workflow. Buffer EN stabilizes biological cells and facilitates binding of cells to the magnetic particles during the capture step. Buffer EAL provides efficient lysis of viruses, bacteria, protozoa, and human cells, while Proteinase K enhances lysis efficiency for more resilient materials such as fungal cells or keratinized tissues. Paramagnetic Beads A enable species-independent cell capture and the removal of cellular debris following heat lysis, resulting in clean DNA extracts suitable for downstream molecular analysis.

Reagents are stored at 2–8 °C and remain stable until the indicated expiry date. After first use, the kit can be used for up to three months, ensuring reliable performance throughout routine laboratory workflows.

Supported Targets

EUROArray SwiftX-traction supports DNA extraction from:

• Human epithelial cells

• Gram-positive and Gram-negative bacteria

• Parasitic protozoa

• Fungi

• Viruses

Compatible Sample Types

Validated specimen types include:

Solid specimens

• Skin dandruff

• Hair bulbs

• Nail shavings

• Dry swabs

• Culture material

• FFPE tissue sections

Liquid specimens

• Urine

• Liquid-based cytology media

These specimen types are commonly used in molecular diagnostic workflows and are compatible with EUROArray assays.

Simple and Streamlined Protocol

EUROArray SwiftX-traction supports several extraction workflows depending on the sample type.

Protocol 1 | Direct extraction from solid specimens

1. Place sample material in a microcentrifuge tube

2. Add Buffer EAL

3. Add Proteinase K and Beads A

4. Incubate at 60 °C for 10 minutes

5. Incubate at 95 °C for 10 minutes

6. Place tube in a magnetic stand

7. Use the supernatant directly for EUROArray analysis

Protocol 2 | Cell capture and extraction from urine

1. Mix urine with Buffer EN and Beads A

2. Incubate 3 minutes at room temperature

3. Capture particles using a magnetic stand and discard supernatant

4. Add Buffer EAL and resuspend beads

5. Incubate 10 minutes at 95 °C

6. Place tube in magnetic stand and collect supernatant for analysis

Protocol 3 | Cell capture and extraction from liquid-based cytology media

1. Combine cytology sample with Buffer EN and Beads A

2. Incubate 3 minutes at room temperature

3. Capture cells using a magnetic stand and discard supernatant

4. Perform an additional wash with Buffer EN

5. Add Buffer EAL and resuspend beads

6. Incubate 10 minutes at 95 °C

7. Place tube in magnetic stand and use supernatant for EUROArray analysis

Extracted DNA can be stored for up to 7 days at 4–8 °C or up to 4 weeks at −20 °C if not immediately used for analysis.