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Microblot-Array Bordetella IgA
Infectious Serology · Bordetella MBA
TestLine Distributed by Goffin Diagnostics

Microblot-Array Bordetella IgA

The Microblot-Array assay is intended for the diagnosis of Bordetella pertussis and Bordetella parapertussis infection using IgA antibodies in human serum or plasma in the general population. The qualitative, semi-quantitative and quantitative automated assay is designed for professional use in a laboratory.

Microblot Array CE-IVD TestLine 1 Package (48 Tests)
Technique
Microblot Array
Brand
TestLine
Packaging
1 Package (48 Tests)
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Product details

This kit in detail

Description

  • Used Antigen: combination of selected parts of the specific antigens of Bordetela pertussis (PT, FHA, ACT, TCF), and Bordetella parapertussis (Pertactin, FimN EntA).
  • Recombinant antigens are applied in the form of microdots (spots) in triplets to a nitrocellulose membrane in a microtiter well format.
  • If specific antibodies are present in the sample, they will bind to the respective antigens.
  • The complex is labelled with Conjugate and detected through a colour reaction with substrate (BCIP/NBT).
  • The kit enables 48 tests.
  • Short incubation periods, total assay time: approximately 1.5 h.
  • Each individual test includes control spots, which serves to check functionality and sensitivity of the sets, and also the spots for checking the presence of the conjugate.
  • Positive controls included enabling validation of the tests.
  • For quantitative evaluation the wells contain calibration spots.
  • All reagents supplied are ready to use.
  • The kit may be used sequentially for smaller batches of samples (reagents are provided in sufficient quantities).
  • Evaluation using Microblot-Array reader and the appropriate software.

Application

  • Differential diagnostics of Bordetella pertussis and parapertussis.
  • Confirmatory method for ELISA and/or agglutination reaction.

Brief assay procedure

  1. Dilute samples serum/plasma (1:51).
  2. Pipette diluted samples.
  3. Incubate at room temperature for 30 min.
  4. Wash 3 times for 5 min.
  5. Add Conjugate.
  6. Incubate at room temperature for 30 min.
  7. Wash 3 times for 5 min.
  8. Add Substrate (BCIP/NPT).
  9. Incubate at room temperature for 15 min.
  10. Wash 2 times for 5 min in distilled water.
  11. Dry and evaluate of test.

Specifications

  • EAN Code: 8595635310614
  • Catalog number: BpAMA48
  • Package size: 48 tests
  • Regulatory status: IVD CE
  • Storage: 2-8 °C
  • Producer: TestLine Clinical Diagnostics s.r.o.
Documentation

Datasheets and regulatory documentation

Everything your quality team needs for evaluation and procurement.

PDF LEAFLET_Microblot Array_full version_ENPDF · download PDF LEAFLET_Microblot-Array overview,pdfPDF · download
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