Respiratory Viral Pathogen Panel Kit

What is in the Respiratory Viral Panel?

The TRUPCR® Respiratory Viral Pathogen Panel Kit is a one-step real-time PCR assay enabling simultaneous detection of a broad range of respiratory viral pathogens. The test is organised across five multiplexed tubes, each using four fluorophores (FAM, HEX/VIC, ROX/Texas Red, Cy5) to detect multiple targets per reaction. This setup provides highly efficient multiplexing and broad coverage of clinically relevant respiratory viruses.

Viral Targets Include

Tube 1:
Human Parechovirus, Human Coronavirus 229E, NL63, OC43, HKU1

Tube 2:
Human Parainfluenza Virus types 1, 2, 3, 4

Tube 3:
Influenza A Virus, Enterovirus, Influenza A (H3N2), Human Metapneumovirus (A/B)

Tube 4:
Pandemic H1 Influenza Virus (pdm09), Influenza B Virus, Influenza C Virus, RNaseP (internal control)

Tube 5:
Human Adenovirus, Respiratory Syncytial Virus A/B, Human Rhinovirus, Human Bocavirus

This comprehensive respiratory viral coverage helps laboratories detect co-infections, support outbreak monitoring, and enable faster clinical decision-making.

Role of Different Targets in Disease

Respiratory Viral Pathogens

Viruses such as Influenza A/B/C, RSV A/B, and seasonal Coronaviruses are among the most common causes of acute respiratory infections worldwide. Parainfluenza viruses frequently affect children and can lead to croup, pneumonia, and bronchiolitis. Human Metapneumovirus and Rhinovirus are major contributors to upper and lower respiratory infections, especially in pediatric and immunocompromised populations. Emerging viruses like Bocavirus and Parechovirus are increasingly recognised in severe pediatric respiratory illness and mixed viral infections.

By covering these diverse viral agents, the panel supports broad differential diagnosis and helps clinicians choose appropriate management strategies more quickly.

About the TRUPCR® Respiratory Viral Pathogen Panel Kit

The TRUPCR® Respiratory Viral Pathogen Panel Kit is a multiplex Real-Time PCR assay developed for accurate and qualitative identification of major respiratory viral pathogens. Key components include:

• Five pre-aliquoted multiplex tubes with validated primer/probe mixes
• Four-channel fluorescence system (FAM, HEX/VIC, ROX, Cy5)
• Human RNaseP gene as an endogenous internal control for sample integrity
• Hot-start polymerase for enhanced assay specificity and sensitivity
• Positive and negative controls for quality assurance
• Broad compatibility with leading Real-Time PCR instruments, including ABI 7500/7500 Fast Dx, QuantStudio® 5, and Bio-Rad® CFX96

Each target is designed against conserved genomic regions, ensuring wide strain detection and minimal cross-reactivity.

Principle and Workflow

1. Sample Collection & Extraction
   Respiratory specimens such as BAL, tracheal aspirates, sputum, and nasopharyngeal aspirates in VTM are processed using standard total nucleic acid extraction procedures.
2. Multiplex PCR Setup
   Extracted nucleic acid is added to five tubes pre-loaded with target-specific master mixes and primer/probe sets.
3. Real-Time Amplification
   PCR runs on compatible qPCR instruments with real-time fluorescence detection across the FAM, HEX/VIC, ROX, and Cy5 channels for accurate multiplex target identification.
4. Result Interpretation
   The RNaseP internal control confirms sample adequacy, and positive controls produce expected Ct values, supporting reliable interpretation and reporting.