Microblot-Array Chlamydia IgG
The Microblot-Array assay is intended for the diagnosis of Chlamydia spp. infection using IgG antibodies in human serum or plasma in the general population. The qualitative, semi-quantitative and quantitative automated assay is designed for professional use in a laboratory.
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This kit in detail
Description
- Used Antigen: combination of selected parts of the specific antigens of Ch. pneumoniae (MOMP, MOMP1, OMP2, OMP4, OMP5, P54), Ch. trachomatis ( MOMP, OMP2, HSP60) and Ch. psittaci (MOMP, OMP2).
- Recombinant antigens are applied in the form of microdots (spots) in triplets to a nitrocellulose membrane in a microtiter well format.
- If specific antibodies are present in the sample, they will bind to the respective antigens.
- The complex is labelled with Conjugate and detected through a colour reaction with substrate (BCIP/NBT).
- The kit enables 96 tests.
- Short incubation periods, total assay time: approximately 1.5 h.
- Each individual test includes control spots, which serves to check functionality and sensitivity of the sets, and also the spots for checking the presence of the conjugate.
- Positive controls included enabling validation of the tests.
- For quantitative evaluation the wells contain calibration spots.
- All reagents supplied are ready to use.
- The kit may be used sequentially for smaller batches of samples (reagents are provided in sufficient quantities).
- Evaluation using Microblot-Array reader and the appropriate software.
Application
- Diagnostic of chlamydia infection.
- Confirmatory test to ELISA.
Brief assay procedure
- Dilute samples serum/plasma (1:51).
- Pipette diluted samples.
- Incubate at room temperature for 30 min.
- Wash 3 times for 5 min.
- Add Conjugate.
- Incubate at room temperature for 30 min..
- Wash 3 times for 5 min.
- Add Substrate (BCIP/NPT).
- Incubate at room temperature for 15 min.
- Wash 2 times for 5 min in distilled water.
- Dry and evaluate of test.
Specifications
- EAN Code: 8595635306952
- Catalog number: CGMA096
- Package size: 96 tests
- Regulatory status: IVD CE2265
- Storage: 2-8 °C
- Producer: TestLine Clinical Diagnostics s.r.o.
Datasheets and regulatory documentation
Everything your quality team needs for evaluation and procurement.
Frequently paired with this kit
Other assays and instruments lab teams add alongside the Microblot-Array Chlamydia IgG.
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