Microblot-Array HSV 1+2 IgM

Description

• Used Antigen: combination of selected parts of the specific antigens of HSV 1+2 (HSV 1+2, gC-1, gC-2, gD-1, gD-2, gG-1, gG-2).
• Recombinant antigens are applied in the form of microdots (spots) in triplets to a nitrocellulose membrane in a microtiter well format.
• If specific antibodies are present in the sample, they will bind to the respective antigens.
• The complex is labelled with Conjugate and detected through a colour reaction with substrate (BCIP/NBT).
• The kit enables 48 tests.
• Short incubation periods, total assay time: approximately 1.5 h.
• Each individual test includes control spots, which serves to check functionality and sensitivity of the sets, and also the spots for checking the presence of the conjugate.
• Positive controls included enabling validation of the tests.
• For quantitative evaluation the wells contain calibration spots.
• All reagents supplied are ready to use.
• The kit may be used sequentially for smaller batches of samples (reagents are provided in sufficient quantities).
• Evaluation using Microblot-Array reader and the appropriate software.

Application

• Diagnostic of Herpes simplex infection.
• Confirmatory test to ELISA.

Brief assay procedure

1. Dilute samples serum/plasma (1:51).
2. Pipette diluted samples.
3. Incubate at room temperature for 30 min.
4. Wash 3 times for 5 min.
5. Add Conjugate.
6. Incubate at room temperature for 30 min.
7. Wash 3 times for 5 min.
8. Add Substrate (BCIP/NPT).
9. Incubate at room temperature for 15 min.
10. Wash 2 times for 5 min in distilled water.
11. Dry and evaluate of test.

Specifications

• EAN Code: 8595635311758
• Catalog number: HSMMA48
• Package size: 48 tests
• Regulatory status: IVD CE2265
• Storage: 2-8 °C
• Producer: TestLine Clinical Diagnostics s.r.o.