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Infectious Serology · Toxocara EIA
TestLine Distributed by Goffin Diagnostics

SmartEIA Toxocara IgG

The immunoenzymatic assay is intended for the diagnosis and monitoring of Toxocara spp. infection using IgG antibodies in human serum or plasma in the general population. The semi-quantitative semi-automated assay is designed for professional use in a laboratory.

SmartEIA | ELISA CE-IVD TestLine 1 Package (96 Tests)
Technique
SmartEIA | ELISA
Brand
TestLine
Packaging
1 Package (96 Tests)
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Quote-based, no public pricing. Add to your quote and our specialists put together a proposal with the right documentation, typically within 24 hours.

Product details

This kit in detail

  • The total assay time is about 1 hour 30 minutes.
  • High sensitivity and specificity of the test.
  • Kit includes CUT-OFF, Positive Control and Negative Control, Calibrators.
  • Semi-quantitative evaluation in the Index of Positivity (IP).
  • Ready-to-use, color-coded components.
  • Single-component substrate.
  • Interchangeable components with the exception of kit specific components (Controls, Conjugate, Plate).
  • Each batch is tested in the National Reference Laboratory.
  • The kit contains the Avidity solution, which enables quantitative determination of bond strength of an antibody antigen complex. Based on this fact, it is possible to distinguish between acute and chronic phase of the disease.

Description

  • Microtitre wells are coated with the excretory-secretory (‘ES’) antigen isolated from the cultured larvae of Toxocara canis.
  • If specific antibodies are present, they bind to the antigen, are labeled by the Conjugate in the following steps and are detected by color reaction with a single component substrate (TMB-Complete).
  • The kit allows 96 tests, including controls in a split microtiter plate with color-coded strips and breakable wells.

Application

  • Laboratory test for the detection of toxocariasis (larva migrans).
  • Post-therapy antibody level monitoring.
  • Differential diagnosis of acute and chronic phase of the disease.

Brief assay procedure

  1. Dilute samples (1:101).
  2. Pipette Controls and diluted Samples.
  3. Incubate at 37°C for 30 min.
  4. Aspirate and wash the wells 5×
  5. Pipette Conjugate.
  6. Incubate at 37°C for 30 min.
  7. Aspirate and wash the wells 5×
  8. Pipette Substrate (TMB-Complete).
  9. Incubate at 37°C for 15 min.
  10. Pipette Stop Solution.
  11. Read color intensity at 450 nm.
  12. Evaluate the test.

SmartEIA kits are specifically designed for automated processing on the Agility® instrument, Dynex Technologies, Inc.

Specifications

  • EAN Code: 8595635305993
  • Catalog number: SK-TcG096
  • Package size: 96 tests
  • Regulatory status: IVD CE
  • Storage: 2-8 °C
  • Producer: TestLine Clinical Diagnostics s.r.o.
Documentation

Datasheets and regulatory documentation

Everything your quality team needs for evaluation and procurement.

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