BLOT-LINE Mycoplasma IgG
The immunoblot assay is intended for the diagnosis of Mycoplasma pneumoniae infection using IgG antibodies in human serum or plasma in the general population. The qualitative and semi-quantitative manual assay is designed for professional use in a laboratory.
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This kit in detail
Description
- Antigen used: combination of selected parts of the specific antigens P1, p30, p116, p65, HMW3, Mgp3.
- Recombinant antigens are transferred to the nitrocellulose membrane (i.e. to the respective BLOT ‐ LINE (BL) strips).
- If specific antibodies are present in the sample, they will bind to the respective antigens.
- The complex is labelled with Conjugate and detected through a colour reaction with substrate (BCIP/NBT).
- The kit enables 20 tests.
- Short incubation periods, total assay time: approximately 1.5 h.
- High sensitivity and specificity.
- All reagents supplied are ready to use.
- The kit may be used sequentially for smaller batches of samples (reagents are provided in sufficient quantities).
- BL strips equipped with conjugate control band and a control band indicating kits functionality and sensitivity.
- Colour identification in compliance with antibody classes.
- Evaluation of results – the intensity of the bands can be evaluated visually or by means of software (Immunoblot Software).
Application
- Diagnostics of M. pneumoniae.
- Confirmatory test to ELISA.
Brief assay procedure
1. Dilute serum/plasma samples (1:51).
2. Pipette diluted samples and controls into the channels of the tray and insert the BL strips.
3. Incubate for 30 min at room temperature using a shaking apparatus.
4. Wash 3 times for 5 min.
5. Add Conjugate.
6. Incubate for 30 min at room temperature using a shaking apparatus.
7. Wash 3 times for 5 min.
8. Add substrate (BCIP/NBT).
9. Incubate for 15 min at room temperature using a shaking apparatus.
10. Wash 2 times for 5 min in distilled water.
11. Dry the strips and evaluate results using the validation strip enclosed or using Immunoblot Software.
Specifications
- EAN Code: 8595635308314
- Catalog number: MyGL20
- Package size: 20 tests
- Regulatory status: IVD CE
- Storage: 2-8 °C
- Delivery time: On request
- Producer: TestLine Clinical Diagnostics s.r.o.
Datasheets and regulatory documentation
Everything your quality team needs for evaluation and procurement.
Frequently paired with this kit
Other assays and instruments lab teams add alongside the BLOT-LINE Mycoplasma IgG.
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