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Blood DNA extraction is streamlined and efficient with the TRUPCR® Blood DNA Extraction Kit, a silica-membrane-based solution designed for the rapid purification of high-quality genomic DNA. Compatible with whole blood, serum, and plasma, this kit offers a reliable method without organic extraction, ensuring maximum yield and purity. Ideal for applications in PCR, qPCR, and NGS, the process includes four key steps: lysis, binding, washing, and elution. With a hands-on time of less than 45 minutes, the kit ensures complete removal of PCR inhibitors, delivering DNA greater than 30 kb in size. Compatible with TRUPCR® JAK2 and Thrombophilia Panel Kits, this extraction solution is ideal for clinical and molecular diagnostic laboratories requiring consistent and reproducible results.
Blood DNA extraction refers to the isolation of genomic DNA from blood samples for downstream molecular applications. This process is essential in clinical diagnostics, research, and molecular genetics. High-quality DNA is critical for accurate PCR, qPCR, sequencing, and mutation detection. The TRUPCR® Blood DNA Extraction Kit enables efficient purification from fresh, frozen, and anticoagulated whole blood with minimal manual intervention.
The reliability of any molecular test—such as mutation analysis, gene expression, or pathogen detection—starts with pure, intact DNA. Contaminants or degraded nucleic acids can result in false negatives or compromised sensitivity. With its high-binding silica membrane, the TRUPCR® Blood DNA Extraction Kit ensures that DNA of sufficient quantity and quality is consistently recovered, minimizing risk and maximizing confidence in results.
This kit is designed for rapid and reliable purification of DNA from whole blood, plasma, or serum. By eliminating phenol-chloroform extraction and lengthy precipitation steps, it ensures safer and faster sample handling. The extracted DNA is of high molecular weight (>30 kb), free from PCR inhibitors, and ready for use in a wide range of downstream processes. It is also compatible with TRUPCR® JAK2 and Thrombophilia Panel Kits for targeted genetic analysis.
The blood DNA extraction process includes:
Lysis – Breaking open blood cells using chaotropic salts and proteinase K.
Binding – Addition of ethanol allows DNA to bind selectively to the silica membrane.
Washing – Removes proteins, salts, and inhibitors.
Elution – DNA is eluted using a low-salt buffer in a 40–50 µl volume.
This process ensures no sample-to-sample cross-contamination and can be performed in under 45 minutes using a standard centrifugation setup.
The TRUPCR® Blood DNA Extraction Kit supports a wide range of diagnostic applications:
Detection of somatic mutations (e.g., JAK2 V617F)
Thrombophilia panel analysis
Genotyping assays for inherited diseases
Preparation for qPCR-based pathogen detection
Compatibility with NGS library preparation
Its performance and reliability make it an ideal fit for molecular diagnostic labs, clinical chemistry departments, and research institutions aiming for reproducibility and speed.
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