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Fecal Virology PCR Kit

Short description

The BIOSYNEX AMPLIQUICK® Fecal Virology Kit is a molecular diagnostic tool for the detection of five gastrointestinal viruses – Rotavirus, Sapovirus, Astrovirus, Norovirus GI/GII and Adenovirus F40/41 – from stool samples by real-time RT-PCR. This reliable kit contains ready-to-use reagents, positive and negative controls, and internal process controls to ensure accurate and efficient virus detection.

Designed for professional laboratories, it allows simultaneous testing with the BIOSYNEX Fecal Bacteriology Kit for comprehensive syndromic diagnosis. This kit simplifies workflow and provides rapid, highly sensitive results for the diagnosis of viral gastroenteritis.

Product highlights

  • Detects five key gastrointestinal viruses with high sensitivity.
  • Ensures accuracy with internal and process controls.
  • Simplifies workflows with ready-to-use reagents.
  • Enables dual testing for comprehensive diagnostics.

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Fecal Virology PCR Kit

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Specifications of the Fecal Virology PCR Kit

Gastroenteritis is a global health problem that affects millions of people every year, especially children under the age of five. It causes diarrhoea, nausea, abdominal pain and severe dehydration, often leading to critical health complications. Viruses such as rotavirus, norovirus GI/GII, sapovirus, astrovirus and adenovirus F40/41 are responsible for up to 70% of cases. Early and accurate identification of these pathogens is essential for timely treatment and control of spread.

Conventional diagnostic methods often lack sensitivity and specificity, resulting in delayed or incomplete diagnoses. BIOSYNEX AMPLIQUICK® Fecal Virology addresses these challenges with an innovative solution for the detection of the major viral causes of gastroenteritis.

A comprehensive diagnostic solution
The BIOSYNEX AMPLIQUICK® Fecal Virology Kit is a molecular diagnostic tool designed for the qualitative detection of five major gastrointestinal viruses. Using advanced real-time RT-PCR technology, the kit amplifies and identifies viral genetic material in stool samples, ensuring accurate and reliable results.

The viruses targeted include Rotavirus, a leading cause of diarrhoea in children, and Norovirus GI/GII, responsible for over 685 million cases annually. Sapovirus, Astrovirus and Adenovirus F40/41 – common in paediatric gastroenteritis – are also detected. With its multiplex PCR approach, the kit can differentiate these pathogens in a single test, providing a complete diagnostic overview.

Designed for laboratory efficiency
Designed for professional laboratories, this kit contains ready-to-use reagents that reduce preparation time and minimise the risk of contamination. It is available in flexible formats, including bulk tubes and prefilled microplates, making it adaptable to different laboratory workflows. Stool samples are processed using BIOSYNEX AMPLIQUICK® Fecal Pretreatment or FecalSwab™ (Copan), ensuring compatibility and easy integration into existing protocols.

Ensure accuracy with built-in controls
The BIOSYNEX AMPLIQUICK® Fecal Virology Kit incorporates robust controls for reliability. The internal control (CIEZ) validates each PCR run, eliminating false negatives caused by inhibition, while the process control (Rp49) ensures sample quality and extraction efficiency. These controls ensure accurate and consistent results, even at low viral loads.

Dual testing for comprehensive diagnosis
For a complete diagnostic approach, the BIOSYNEX AMPLIQUICK® Fecal Virology Kit can be combined with the BIOSYNEX AMPLIQUICK® Fecal Bacteriology Kit. This syndromic capability allows the simultaneous detection of viral and bacterial pathogens from a single stool sample, providing clinicians with a complete picture of the infection and enabling targeted treatment decisions.

Improving patient care
The BIOSYNEX AMPLIQUICK® Fecal Virology Kit provides rapid and reliable detection of viral gastroenteritis, addressing a critical need in clinical diagnostics. Its advanced technology and efficient design help laboratories deliver timely results, improving patient outcomes through faster and more accurate treatment interventions. By detecting multiple pathogens in a single test, this kit is a valuable tool in the fight against gastroenteritis, particularly in paediatric and high-risk populations.

Components of the Fecal Virology PCR Kit
  1. Master Mix A: Targets detection of Rotavirus, Sapovirus, and Astrovirus.
  2. Master Mix B: Targets detection of Norovirus GI/GII and Adenovirus F40/41.
  3. Positive Control: Ensures proper PCR amplification and validates test functionality.
  4. Negative Control: Confirms no contamination in the testing process.
  5. Control in Process: Monitors sample quality and extraction efficiency using the Rp49 sequence.
  6. Master Mix Control: Validates the integrity and performance of the master mixes.
  7. Optical Cap Strips: Seal PCR plates to prevent contamination and ensure accurate fluorescence detection.
  8. Prefilled Microplates (Optional): Dividable 96-well microplates preloaded with master mixes for efficient testing.

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