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Tissue DNA extraction is streamlined and highly efficient with the TRUPCR® Tissue DNA Extraction Kit, designed for rapid isolation of high-purity DNA from animal tissue, cells, bacteria, and blood. The silica membrane–based system utilizes a proteinase K and SDS lysis protocol followed by specific binding, washing, and elution steps. With a flexible elution range and robust contaminant removal, this kit delivers consistent yields of PCR-ready DNA from a wide range of sample types. The protocol eliminates hazardous reagents and supports various molecular applications, including PCR and NGS.
Tissue DNA extraction is the process of isolating high-quality DNA from biological tissues and other complex samples. This is a critical first step in molecular diagnostics, genotyping, and pathogen detection. The TRUPCR® Tissue DNA Extraction Kit offers a reliable, scalable method for isolating both genomic and microbial DNA from diverse sample sources including tissue biopsies, cultured cells, and body fluids.
High-quality DNA is the foundation of accurate PCR, sequencing, and diagnostic workflows. Inconsistent yield, contamination, or inhibitors can compromise test results. The TRUPCR® Tissue DNA Extraction Kit provides the purity, yield, and reproducibility necessary for high-throughput laboratories and clinical diagnostics.
This kit follows a proven 4-step method:
Enzymatic Lysis – A proteinase K/SDS lysis buffer breaks down cell membranes and releases DNA
Binding – DNA binds to a silica membrane under chaotropic conditions with ethanol
Washing – Efficient wash steps remove proteins, salts, and PCR inhibitors
Elution – Purified DNA is eluted in a user-defined volume (20–100 µL)
The process is optimized for recovery of DNA longer than 30 kb and yields highly pure DNA suitable for PCR, real-time PCR, and NGS applications.
Technology: Silica column
Sample Types: Animal tissue, bacterial cultures, blood, body fluids
Applications: PCR, qPCR, NGS, sequencing
Elution Volume: 20–100 µL
Processing: Manual (centrifugation), hands-on time ≤ 1 hour
Yield: High consistency and reproducibility from minimal sample input
All buffers are optimized to ensure complete lysis, high DNA binding, and efficient removal of inhibitors—ensuring DNA of the highest integrity.
The TRUPCR® Tissue DNA Extraction Kit is a versatile solution for:
Pathogen detection from infected tissues
Genomic studies using biopsies or animal models
Cancer research via DNA from tumor tissues
Molecular diagnostics for both host and microbial targets
NGS library prep requiring high-purity DNA
Whether you’re processing clinical biopsies or lab-cultured samples, this kit ensures reproducible and inhibitor-free DNA extraction.
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