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Blood RNA extraction is simplified and highly efficient with the TRUPCR® Blood RNA Extraction Kit, designed to purify high-quality cellular RNA from fresh, anticoagulated whole blood. Utilizing silica column technology and unique buffer systems, the kit ensures complete red cell lysis and selective RNA binding from white blood cells. The protocol eliminates the need for hazardous organic solvents like phenol or chloroform. Instead, RNA is safely and effectively isolated in under 1 hour with minimal hands-on time. The kit is compatible with key downstream molecular applications, including PCR, RT-PCR, cDNA synthesis, and NGS. With consistent yield, minimal RNase contamination, and compatibility with TRUPCR® BCR-ABL1, AML, and ALL panel kits, this solution is ideal for clinical and molecular laboratories requiring reliable RNA purification.
Blood RNA extraction refers to the process of isolating total RNA—especially from leukocytes in whole blood—for downstream molecular applications. Unlike DNA, RNA is more fragile and sensitive to degradation. Therefore, extraction methods must be fast, efficient, and gentle. The TRUPCR® kit enables the purification of high-quality RNA from whole blood using silica spin columns and proprietary buffers, offering an optimal workflow for RT-PCR and gene expression analysis.
RNA extracted from whole blood is essential for diagnosing hematological malignancies, monitoring minimal residual disease, and studying gene expression. It is particularly valuable in detecting fusion genes (e.g., BCR-ABL1) and in real-time PCR applications. The TRUPCR® Blood RNA Extraction Kit offers complete removal of inhibitors such as hemoglobin and heparin, which are common in blood-derived RNA, ensuring that the RNA is ready-to-use and yields reproducible results.
This kit provides an advanced alternative to traditional RNA isolation methods like ultracentrifugation or phenol-chloroform extraction. It is designed for fresh samples (not frozen), and the protocol includes:
Erythrocyte lysis
Leukocyte enrichment
RNase inactivation and lysis
RNA binding and purification on a silica membrane
Elution in 30–50 µl RNase-free water
Total RNA recovered is high-quality, with A260/A280 values exceeding 1.9, suitable for both qualitative and quantitative molecular workflows.
The blood RNA extraction process involves:
Selective lysis of red blood cells using proprietary buffer
Recovery of white blood cells via centrifugation
Lysis of leukocytes under denaturing conditions that degrade RNases
RNA binding to the silica membrane
Washing to remove proteins, salts, and contaminants
Elution of purified RNA
This workflow ensures a fast and consistent preparation of RNA in less than one hour and is designed to prevent cross-contamination while enabling parallel processing of multiple samples.
The TRUPCR® Blood RNA Extraction Kit supports a wide range of molecular diagnostics:
Detection of fusion transcripts like BCR-ABL1 (chronic myeloid leukemia)
Acute leukemia panels (e.g., AML, ALL)
Monitoring treatment efficacy through gene expression profiling
RT-PCR-based viral detection
Use in clinical and translational research studies
Its reliability and simplicity make it ideal for use in routine diagnostics, clinical research labs, and high-throughput molecular screening programs.
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