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MTB DNA extraction is made efficient and reliable with the TRUPCR® MTB DNA Extraction Kit, designed to purify Mycobacterium tuberculosis DNA from sputum, BAL, PUS, CSF, and tissue samples. Using advanced silica column technology and a heat lysis protocol tailored for M. tuberculosis’s tough cell wall, the kit ensures maximum DNA yield with minimal contamination. The four-step process—heat lysis, binding, washing, and elution—produces PCR-ready DNA suitable for TB diagnostics and resistance testing. Compatible with TRUPCR®’s downstream MTB detection kits, this extraction kit streamlines molecular workflows for infectious disease labs.
MTB DNA extraction refers to isolating high-quality DNA from Mycobacterium tuberculosis in clinical specimens. Due to its waxy, mycolic acid-rich cell wall, MTB is one of the most difficult pathogens to lyse. Accurate PCR-based diagnosis of tuberculosis depends on efficient DNA extraction. This kit enables the robust lysis and purification needed for successful downstream detection of MTB DNA, even in low-bacterial-load specimens.
Timely and sensitive detection of MTB is critical due to its long doubling time and high infectivity. Delayed diagnosis often leads to increased transmission and poor clinical outcomes. Molecular diagnostics like PCR have become essential tools, but their effectiveness depends on the quality of extracted DNA. The TRUPCR® MTB DNA Extraction Kit ensures recovery of sufficient DNA, even from difficult matrices like sputum or tissue.
The kit is based on silica column spin technology, optimized specifically for the tough cell wall of M. tuberculosis. The protocol includes:
Heat Lysis: Breaks down mycolic acid–rich bacterial walls
Binding: DNA binds to a silica membrane in the presence of chaotropic salts and ethanol
Washing: Multiple wash steps remove proteins, inhibitors, and contaminants
Elution: DNA is eluted in 40–50 µL of low-salt buffer
The result: highly pure DNA ideal for conventional PCR and real-time PCR applications.
The TRUPCR® MTB DNA Extraction Kit follows a reliable, contamination-free workflow:
Sample Types: Sputum, BAL, PUS, CSF, tissues, urine, and other body fluids
Lysis Method: Heat lysis + chaotropic buffer
Purification: Silica membrane spin columns
Processing Time: < 1 hour (manual centrifugation)
Output: 40–50 µL of purified MTB DNA
No phenol, no chloroform—just clean, efficient MTB DNA extraction.
This kit is an essential tool in:
TB diagnostics using PCR or qPCR
Detection of Rifampicin resistance when used with TRUPCR® Rifampicin Resistance MTB Kit
Nested PCR workflows for detecting low-copy MTB in complex samples
Public health surveillance, infection control, and antimicrobial resistance monitoring
Its validated compatibility with TRUPCR® MTBC Onestep Nested Kit and resistance panels ensures seamless workflow integration.
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