The BIOSYNEX AMPLIQUICK® Respiratory Triplex Kit is an advanced RT-PCR diagnostic tool designed for the simultaneous detection of SARS-CoV-2, Influenza A and B, and Respiratory Syncytial Virus (RSV). Using a single nasopharyngeal swab, the kit enables healthcare professionals to accurately identify infections that often share similar symptoms such as fever, cough and fatigue.

Critical role in public health
Respiratory diseases caused by these pathogens are a major public health challenge. COVID-19, caused by SARS-CoV-2, has a mortality rate of about 2%. Influenza A and B are responsible for nearly half a million deaths annually, and RSV is a leading cause of respiratory infection in young children. This kit allows rapid differentiation between these diseases, supporting timely treatment decisions and isolation measures, particularly during outbreaks and seasonal epidemics.

Reliable RT-PCR technology
The kit uses real-time RT-PCR technology in two steps. First, viral RNA and an internal human RNA control are converted to complementary DNA (cDNA). Next, specific primers and fluorophore-labelled probes amplify target genes, including two genes from SARS-CoV-2 (E and RdRp), Influenza A (M), Influenza B (HA) and RSV (M). Internal controls ensure the reliability of the sample collection, RNA extraction and amplification processes, preventing false negatives.

Easy to use design
The kit comes in prefilled 96-well microtiter plates containing all necessary reagents, including Master Mix, primers, probes, Taq polymerase and buffers. This ready-to-use format simplifies laboratory workflow, reducing preparation time while maintaining high diagnostic accuracy.

Broad application and compatibility
The kit has been validated for use with RNA extracted from nasopharyngeal swabs and is compatible with the BIOSYNEX AMPLIQUICK® Sample Collection Kit and the BIOSYNEX AMPLIQUICK® Lysis Kit. This compatibility ensures seamless integration into diagnostic workflows, making it a versatile tool for different laboratory settings.

Professional Utility
Designed for use by trained laboratory professionals, the BIOSYNEX AMPLIQUICK® Respiratory Triplex Kit delivers fast, sensitive and reliable results. It is an essential tool for identifying both asymptomatic and symptomatic cases, aiding in effective patient management and infection control.