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SARS-CoV-2 PCR Assay

Short description

The ViennaLab SARS-CoV-2 RealFast Assay is a one-step real-time reverse transcription PCR (RT-PCR) assay that is highly sensitive and specifically detects two viral target genes (N and RdRP/ORF1ab), making it the gold standard for rapid and sensitive SARS-CoV-2 identification. It is essential to test individuals without clinical or subclinical symptoms who may have been exposed to SARS-CoV-2, and the RealFast Assay allows for highly sensitive detection with a low limit of detection (LoD: 10 copies per reaction). Additionally, the assay has passed external quality assessment schemes and is compatible with a wide range of real-time PCR instruments.

Product highlights

  • Multiplex one-tube reaction assay
  • Two separate virus specific target genes
  • High sensitivity (LoD: 10 copies per reaction)
  • Compatible with most real-time PCR devices

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SARS-CoV-2 PCR Assay

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Specifications of the SARS-CoV-2 PCR Assay

Introducing the ViennaLab SARS-CoV-2 RealFast Assay – the gold standard in rapid and highly sensitive SARS-CoV-2 identification. SARS-CoV-2 is a positive sense, single stranded RNA respiratory virus that emerged in late 2019, and has since become a global pandemic. With the silent spread of the virus, it’s essential to test individuals without clinical or subclinical symptoms who may have been exposed to control the pandemic locally, regionally, and globally.

This highly sensitive assay allows for the detection of two viral target genes (N and RdRP/ORF1ab), specific to SARS-CoV-2, in a one-tube reaction. The RealFast Assay is a multiplex one-tube reaction assay that includes a human ACTB gene-detecting control, with a high sensitivity of LoD: 10 copies per reaction, ensuring accurate results. Additionally, the assay has successfully passed external quality assessment schemes and is compatible with a wide range of real-time PCR instruments.

As several countries temporarily reduce the number of daily new cases, robust diagnostic tools that facilitate the efficient control of new outbreaks remain highly demanded. The RealFast Assay provides a quick and sensitive testing method using real-time reverse transcription PCR technology, ensuring accurate detection of current infections.

Components of the SARS-CoV-2 PCR Assay
  1. RNA Extraction Kit: A kit containing all the reagents necessary for the extraction of RNA from a respiratory sample, including RNA extraction buffer, proteinase K, and magnetic beads.
  2. PCR Mix: A ready-to-use mix containing all the reagents necessary for the PCR amplification of the SARS-CoV-2 gene regions of interest.
  3. SARS-CoV-2 Primer Mix: A mix of primers specific for the SARS-CoV-2 gene regions of interest.
  4. Probe Mix: A mix of probes specific for the detection of SARS-CoV-2 RNA.
  5. Control RNA: A synthetic RNA that is added to the sample as an internal control to ensure that the assay is working correctly.
  6. DNase/RNase-free Water: Water that has been treated to remove contaminating DNases and RNases, which can degrade the extracted RNA.

 

The kit also includes instructions for use, as well as a protocol for the detection of SARS-CoV-2 RNA using real-time PCR.

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